Analysis of Genetic Linkage of HIV From Couples Enrolled in the HIV Prevention Trials Network 052 Trial

被引：89

作者：

Eshleman, Susan H. ^{[1
]}

Hudelson, Sarah E. ^{[1
]}

Redd, Andrew D. ^{[2
]}

Wang, Lei ^{[3
]}

Debes, Rachel ^{[4
]}

Chen, Ying Q. ^{[3
]}

Martens, Craig A. ^{[5
]}

Ricklefs, Stacy M. ^{[5
]}

Selig, Ethan J. ^{[5
]}

Porcella, Stephen F. ^{[5
]}

Munshaw, Supriya ^{[6
]}

Ray, Stuart C. ^{[6
]}

Piwowar-Manning, Estelle ^{[1
]}

McCauley, Marybeth ^{[7
]}

Hosseinipour, Mina C. ^{[8
]}

Kumwenda, Johnstone ^{[9
]}

Hakim, James G. ^{[10
]}

Chariyalertsak, Suwat ^{[11
]}

de Bruyn, Guy ^{[12
]}

Grinsztejn, Beatriz ^{[13
]}

Kumarasamy, Nagalingeswaran ^{[14
]}

Makhema, Joseph ^{[15
]}

Mayer, Kenneth H. ^{[16
]}

Pilotto, Jose ^{[17
,18
]}

Santos, Breno R. ^{[20
]}

Quinn, Thomas C. ^{[2
]}

Cohen, Myron S. ^{[19
]}

Hughes, James P. ^{[3
,4
]}

机构：

[1] Johns Hopkins Univ, Sch Med, Dept Pathol, Baltimore, MD 21205 USA

[2] NIAID, Immunoregulat Lab, Div Intramural Res, NIH, Baltimore, MD USA

[3] Fred Hutchinson Canc Res Ctr, Vaccine & Infect Dis Div, Seattle, WA 98104 USA

[4] Univ Washington, Dept Biostat, Seattle, WA 98195 USA

[5] NIAID, Genom Unit, Res Technol Sect, Rocky Mt Labs,Div Intramural Res,NIH, Hamilton, MT USA

[6] Johns Hopkins Univ, Sch Med, Dept Pathol, Baltimore, MD 21205 USA

[7] FHI 360, Hlth & Dev Sci Div, Arlington, VA USA

[8] Kamuzu Cent Hosp, UNC Project, Lilongwe, Malawi

[9] Coll Med, Blantyre, Malawi

[10] Univ Zimbabwe, Dept Med, Harare, Zimbabwe

[11] Chiang Mai Univ, Res Inst Hlth Sci, Chiang Mai, Thailand

[12] Univ Witwatersrand, Perinatal HIV Res Unit, Johannesburg, South Africa

[13] Inst Pesquisa Clin Evandro Chagas Fiocruz, Lab Pesquisa Clin DST AIDS, Rio De Janeiro, Brazil

[14] YRG CARE Med Ctr, Madras, Tamil Nadu, India

[15] Botswana Harvard Sch Publ Hlth AIDS Initiat Partn, Gaborone, Botswana

[16] Fenway Hlth, Boston, MA USA

[17] Hosp Geral Nova Iguacu, Rio De Janeiro, Brazil

[18] Lab AIDS & Imunol Mol IOC Fiocruz, Rio De Janeiro, Brazil

[19] Univ N Carolina, Dept Med, Chapel Hill, NC USA

[20] Hosp Nossa Senhora da Conceicao, Porto Alegre, RS, Brazil

来源：

JOURNAL OF INFECTIOUS DISEASES | 2011年 / 204卷 / 12期

关键词：

MOLECULAR EPIDEMIOLOGY; IDENTIFICATION; EVOLUTIONARY; TRANSMISSION;

D O I：

10.1093/infdis/jir651

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Methods. Linkage was assessed using phylogenetic analysis of HIV pol sequences and Bayesian analysis of genetic distances between pol sequences from index-partner pairs and controls. Selected samples were also analyzed using next-generation sequencing (env region). Results. In 29 of the 38 (76.3%) cases analyzed, the index was the likely source of the partner's HIV infection (linked). In 7 cases (18.4%), the partner was most likely infected from a source other than the index participant (unlinked). In 2 cases (5.3%), linkage status could not be definitively established. Conclusions. Nearly one-fifth of the seroconversion events in HPTN 052 were unlinked. The association of early ART and reduced HIV transmission was stronger when the analysis included only linked events. This underscores the importance of assessing the genetic linkage of HIV seroconversion events in HIV prevention studies involving serodiscordant couples.

引用

页码：1918 / 1926

页数：9

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