The Bacillus subtilis 168 chromosome from sspE to katA

被引:5
作者
Cummings, NJ [1 ]
Connerton, IF [1 ]
机构
[1] INST FOOD RES,DEPT FOOD MACROMOL SCI,READING RG6 6BZ,BERKS,ENGLAND
来源
MICROBIOLOGY-UK | 1997年 / 143卷
关键词
Bacillus subtilis; genome sequencing; glutamate-1-semialdehyde aminotransferase; rrnD operon; trnD cluster;
D O I
10.1099/00221287-143-6-1855
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
We have cloned and sequenced a 24.5 kb region of the Bacillus subtilis 168 chromosome spanning the sspE and katA genes. The region contains a ribosomal RNA operon, rrnD, a tRNA gene set, trnD and 17 ORFs, 16 with putative ribosome-binding sites. Four of the ORFs (ORF2, ORF14, ORF16 and ORF17) match to known B. subtilis genes (sspE, thiA, senS and katA). Eight of the remaining ORF products show similarities with proteins present in the databases, including an ATP-binding transport protein, a glutamate-1-semialdehyde aminotransferase, a thiol-specific antioxidant protein, a mitomycin radical oxidase and a ferric uptake regulation protein.
引用
收藏
页码:1855 / 1859
页数:5
相关论文
共 14 条
[1]  
ALTSCHUL SF, 1990, J MOL BIOL, V215, P403, DOI 10.1006/jmbi.1990.9999
[2]   Computerized genetic map of Bacillus subtilis [J].
Biaudet, V ;
Samson, F ;
Anagnostopoulos, C ;
Ehrlich, SD ;
Bessieres, P .
MICROBIOLOGY-SGM, 1996, 142 :2669-2729
[3]   A COMPREHENSIVE SET OF SEQUENCE-ANALYSIS PROGRAMS FOR THE VAX [J].
DEVEREUX, J ;
HAEBERLI, P ;
SMITHIES, O .
NUCLEIC ACIDS RESEARCH, 1984, 12 (01) :387-395
[4]  
GRIMM B, 1991, MOL GEN GENET, V225, P1
[5]   CLONING, NUCLEOTIDE SEQUENCING, AND GENETIC-MAPPING OF THE GENE FOR SMALL, ACID-SOLUBLE SPORE PROTEIN-GAMMA OF BACILLUS-SUBTILIS [J].
HACKETT, RH ;
SETLOW, P .
JOURNAL OF BACTERIOLOGY, 1987, 169 (05) :1985-1992
[6]   THE BACILLUS-SUBTILIS HEMAXCDBL GENE-CLUSTER, WHICH ENCODES ENZYMES OF THE BIOSYNTHETIC-PATHWAY FROM GLUTAMATE TO UROPORPHYRINOGEN-III [J].
HANSSON, M ;
RUTBERG, L ;
SCHRODER, I ;
HEDERSTEDT, L .
JOURNAL OF BACTERIOLOGY, 1991, 173 (08) :2590-2599
[7]  
ITAYA M, 1991, J MOL BIOL, V169, P1911
[8]  
Maniatis T., 1982, MOL CLONING
[9]  
MCLAUGHLIN JR, 1981, J BIOL CHEM, V256, P1283
[10]  
RAUE HA, 1977, MOL GEN GENET, V156, P185