Calculation of a gap restoration in the membrane skeleton of the red blood cell:: Possible role for myosin II in local repair

被引:11
作者
Cibert, C
Prulière, G
Lacombe, C
Deprette, C
Cassoly, R
机构
[1] Univ Paris 06, Dev Biol Lab, Inst Jacques Monod, CNRS,UMR 7592, F-75005 Paris, France
[2] Univ Paris 07, F-75005 Paris, France
[3] CNRS, URA 671, Stn Zool Marine, F-06234 Villefranche Sur Mer, France
[4] Univ Paris 06, Inst Jacques Monod, Lab Bioactivat Peptides, CNRS,UMR 7592, F-75005 Paris, France
[5] Univ Paris 06, Lab Biol Cellulaire Membranes, Inst Jacques Monod, CNRS,UMR 7592, Paris, France
关键词
D O I
10.1016/S0006-3495(99)77280-2
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
Human red blood cells contain all of the elements involved in the formation of nonmuscle actomyosin II complexes (V. M. Fowler. 1986. J. Cell. Biochem. 31:1-9; 1996. Curr. Opin. Cell Biol. 8:86-96). No clear function has yet been attributed to these complexes. Using a mathematical model for the structure of the red blood cell spectrin skeleton (M. J. Saxton. 1992. J. Theor. Biol. 155:517-536), we have explored a possible role for myosin II bipolar minifilaments in the restoration of the membrane skeleton, which may be locally damaged by major mechanical or chemical stress. We propose that the establishment of stable links between distant antiparallel actin protofilaments after a local myosin II activation may initiate the repair of the disrupted area. We show that it is possible to define conditions in which the calculated number of myosin II minifilaments bound to actin protofilaments is consistent with the estimated number of myosin II minifilaments present in the red blood cells. A clear restoration effect can be observed when more than 50% of the spectrin polymers of a defined area are disrupted. It corresponds to a significant increase in the spectrin density in the protein free region of the membrane. This may be involved in a more complex repair process of the red blood cell membrane, which includes the vesiculation of the bilayer and the compaction of the disassembled spectrin network.
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页码:1153 / 1165
页数:13
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