Real-time imaging of tunable adenosine 5-triphosphate release from an MCM-41-type mesoporous silica nanosphere-based delivery system

被引：60

作者：

Gruenhagen, JA

Lai, CY

Radu, DR

Lin, VSY

Yeung, ES ^{[1
]}

机构：

[1] Iowa State Univ, Ames Lab, US DOE, Ames, IA 50011 USA

[2] Iowa State Univ, Dept Chem, Ames, IA 50011 USA

来源：

APPLIED SPECTROSCOPY | 2005年 / 59卷 / 04期

关键词：

ATP sensing; luciferase chemiluminescence; MCM-41; capped mesoporous silica; poly(amido amine) dendrimer; controlled release; fluorescence imaging;

D O I：

10.1366/0003702053641513

中图分类号：

TH7 [仪器、仪表];

学科分类号：

0804 ; 080401 ; 081102 ;

摘要：

We studied a mesoporous silica nanosphere (MSN) material with tunable release capability for drug delivery applications. We employed luciferase chemiluminescence imaging to investigate the kinetics and mechanism of the adenosine 5-triphosphate (ATP) release with various disulfide-reducing agents as uncapping triggers. ATP molecules were encapsulated within the MSNs by immersing dry nanospheres in aqueous solutions of ATP followed by capping of the mesopores with chemically removable caps, such as cadmium sulfide (CdS) nanoparticles and poly(amido amine) dendrimers (PAMAM), via a disulfide linkage. By varying the chemical nature of the "cap" and "trigger" molecules in our MSN system, we discovered that the release profiles could indeed be regulated in a controllable fashion.

引用

页码：424 / 431

页数：8

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