Leuconostoc holzapfelii sp nov., isolated from Ethiopian coffee fermentation and assessment of sequence analysis of housekeeping genes for delineation of Leuconostoc species

被引：82

作者：

De Bruyne, Katrien ^{[1
]}

Schillinger, Ulrich ^{[2
]}

Caroline, Lily ^{[2
]}

Boehringer, Benjamin ^{[2
]}

Cleenwerck, Ilse ^{[3
]}

Vancanneyt, Marc ^{[3
]}

De Vuyst, Luc ^{[4
]}

Franz, Charles M. A. P. ^{[2
]}

Vandamme, Peter ^{[1
]}

机构：

[1] Univ Ghent, Microbiol Lab, B-9000 Ghent, Belgium

[2] Inst Hyg & Toxicol, Fed Res Ctr Nutr Food, D-76131 Karlsruhe, Germany

[3] Univ Ghent, BCCM LMG Bacteria Collect, B-9000 Ghent, Belgium

[4] Vrije Univ Brussels, Dept Appl Biol Sci & Engn, Res Grp Ind Microbiol & Food Biotechnol IMDO, B-1050 Brussels, Belgium

来源：

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY | 2007年 / 57卷

关键词：

D O I：

10.1099/ijs.0.65292-0

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A Gram-positive, ovoid lactic acid bacterium, strain LMG 23990(T), was isolated from Ethiopian coffee fermentation. 16S rRNA gene sequence analysis indicated that the novel strain belongs to the genus Leuconostoc, with Leuconostoc citreum and Leuconostoc lactis as the closest neighbours (99.6 and 99.0 % 16S rRNA gene sequence similarity, respectively). Genotypic fingerprinting by fluorescent amplified fragment length polymorphism, whole-cell protein electrophoresis, DNA-DNA hybridizations, comparative sequence analysis of pheS, rpoA, atpA, and physiological and biochemical tests allowed us to differentiate strain LMG 23990(T) from all established Leuconostoc species. Strain LMG 23990(T) (=CCUG 54536(T)) therefore represents a novel species, for which the name Leuconostoc holzapfelii sp. nov. is proposed.

引用

页码：2952 / 2959

页数：8

共 36 条

[1]

[Anonymous], CHEM METHODS PROKARY

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