3D-Printed Microfluidic Device for the Detection of Pathogenic Bacteria Using Size-based Separation in Helical Channel with Trapezoid Cross-Section

被引：217

作者：

Lee, Wonjae ^{[1
]}

Kwon, Donghoon ^{[1
]}

Choi, Woong ^{[2
]}

Jung, Gyoo Yeol ^{[2
]}

Jeon, Sangmin ^{[1
]}

机构：

[1] Pohang Univ Sci & Technol POSTECH, Dept Chem Engn, Pohang, Gyeongbuk, South Korea

[2] Pohang Univ Sci & Technol POSTECH, Sch Interdisciplinary Biosci & Bioengn, Pohang, South Korea

来源：

SCIENTIFIC REPORTS | 2015年 / 5卷

基金：

新加坡国家研究基金会;

关键词：

ESCHERICHIA-COLI O157-H7; MAGNETIC NANOPARTICLES; RAPID DETECTION; PARTICLE; FRACTIONATION; FLOW; STRATEGIES;

D O I：

10.1038/srep07717

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

A facile method has been developed to detect pathogenic bacteria using magnetic nanoparticle clusters (MNCs) and a 3D-printed helical microchannel. Antibody-functionalized MNCs were used to capture E. coli (EC) bacteria in milk, and the free MNCs and MNC-EC complexes were separated from the milk using a permanent magnet. The free MNCs and MNC-EC complexes were dispersed in a buffer solution, then the solution was injected into a helical microchannel device with or without a sheath flow. The MNC-EC complexes were separated from the free MNCs via the Dean drag force and lift force, and the separation was facilitated in the presence of a sheath flow. The concentration of the E. coli bacteria was determined using a light absorption spectrometer, and the limit of detection was found to be 10 cfu/mL in buffer solution and 100 cfu/mL in milk.

引用

页数：6

共 35 条

[31] Quantitative and simultaneous detection of four foodborne bacterial pathogens with a multi-channel SPR sensor [J].