Extracellular matrix tenascin-X in combination with vascular endothelial growth factor B enhances endothelial cell proliferation

Background: An extracellular matrix tenascin-X (TNX) is highly expressed in muscular tissues, especially heart and skeletal muscle, and is also prominent around blood vessels. The precise in vivo role of TNX remains to be elucidated. To identify proteins that interact with TNX in the extracellular environment, we searched for TNX-binding proteins using a yeast two-hybrid system, Results: We used mouse TNX-specific fibronectin type III repeats (mTNX/FNIII13-25) as a bait for the screening. We found that vascular endothelial growth factor B (VEGF-B) binds to mTNX/FNIII13-25. This interaction was confirmed by pull-down assays and co-immunoprecipitation assays. The full-length mTNX, as well as mTNX/FNIII13-25, interacted with both alternative splice isoforms VEGF-B-186 and VEGF-B-167 Furthermore, the full-length mTNX also bound to VEGF-A, The minimal region of TNX that interacts with VEGF-B was marred to the FNIII repeats (FNIII13-25) but not to the other characteristic domains of TNX, The TNX-binding site of VEGF-B was located in the N-terminal 115-amino acid region. mTNX/FNIII13-25 did not prevent the interaction of VEGF-B with VEGFR-1 (VEGF receptor 1), and VEGF-B could simultaneously bind to both mTNX/FNTII13-25 and VEGFR-1, A conditioned medium from transfected 293T cells coexpressing full-length TNX and VEGF-B could promote DNA synthesis in bovine endothelial cells in which VEGFR-1 were expressed. VEGFR-1 phosphorylation triggered by VEGF-B-186 were increased in cells plated with mTNX/FNIII13-25 or full-length mTNX, compared with cells plated with VEGF-B-186 alone. Conclusion: TNX interacts with VEGF-B and enhances the ability of VEGF-B to stimulate cell, proliferation. This enhanced mitogenecity is caused by increased signals mediated by the VEGFR-1 receptor, This finding suggests a role for TNX in the regulation of the development of blood vessels such as vasculogenesis and angiogenesis.