Biocontrol by phenazine-1-carboxamide-producing Pseudomonas chlororaphis PCL1391 of tomato root rot caused by Fusarium oxysporum f. sp. radicis-lycopersici

被引:241
作者
Chin-A-Woeng, TFC
Bloemberg, GV
van der Bij, AJ
van der Drift, KMGF
Schripsema, J
Kroon, B
Scheffer, RJ
Keel, C
Bakker, PAHM
Tichy, HV
de Bruijn, FJ
Thomas-Oates, JE
Lugtenberg, BJJ
机构
[1] Leiden State Univ, Inst Mol Plant Sci, Clusius Lab, NL-2333 AL Leiden, Netherlands
[2] Univ Utrecht, Dept Mass Spectrometry, FAFC Wentgebouw, NL-3584 CA Utrecht, Netherlands
[3] Univ Fed Rio de Janeiro, Ctr Ciencias Saude, BR-21941590 Rio De Janeiro, Brazil
[4] Novartis Seeds BV, NL-1600 AA Enkhuizen, Netherlands
[5] Univ Lausanne, Lab Biol Microbienne, CH-1015 Lausanne, Switzerland
[6] Univ Utrecht, Dept Plant Ecol & Evolutionary Biol, Sect Plant Pathol, NL-3508 TB Utrecht, Netherlands
[7] TUV Energie & Umwelt GMBH, Niederlassung Freiburg, D-79108 Freiburg, Germany
[8] Michigan State Univ, US DOE, Plant Res Lab, E Lansing, MI 48824 USA
关键词
microbiological control;
D O I
10.1094/MPMI.1998.11.11.1069
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Seventy bacterial isolates from the rhizosphere of tomato were screened for antagonistic activity against the tomato foot and root rot-causing fungal pathogen Fusarium oxysporum f. sp. radicis-lycopersici, One isolate, strain PCL1391, appeared to be an efficient colonizer of tomato roots and an excellent biocontrol strain in an F. oxysporum/tomato test system. Strain PCL1391 was identified as Pseudomonas chlororaphis and further characterization showed that it produces a broad spectrum of antifungal factors (AFFs), including a hydrophobic compound, hydrogen cyanide, chitinase(s), and protease(s). Through mass spectrometry and nuclear magnetic resonance, the hydrophobic compound was identified as phenazine-l-carboxamide (PCN). We have studied the production and action of this AFF both in vitro and in vivo. Using a PCL1391 transposon mutant, with a lux reporter gene inserted in the phenazine biosynthetic operon (phz), we showed that this phenazine biosynthetic mutant was substantially decreased in both in vitro antifungal activity and biocontrol activity. Moreover, with the same mutant it was shown that the phz biosynthetic operon is expressed in the tomato rhizosphere. Comparison of the biocontrol activity of the PCN-producing strain PCL1391 with those of phenazine-l-carboxylic acid (PCA)producing strains P. fluorescens 2-79 and P. aureofaciens 30-84 showed that the PCN-producing strain is able to suppress disease in the tomato/F. oxysporum system.
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页码:1069 / 1077
页数:9
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