Analysis of pharmaceutical preparations containing local anesthetics by micellar liquid chromatography and spectrophotometric detection

被引：21

作者：

Escuder-Gilabert, L ^{[1
]}

Sagrado, S ^{[1
]}

Villanueva-Camañas, RM ^{[1
]}

Medina-Hernandez, MJ ^{[1
]}

机构：

[1] Univ Valencia, Dept Quim Analit, E-46100 Valencia, Spain

来源：

CHROMATOGRAPHIA | 1999年 / 49卷 / 1-2期

关键词：

column-liquid chromatography; micellar liquid chromatograph; local anesthetic; pharmaceuticals preparations;

D O I：

10.1007/BF02467193

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

An HPLC procedure for the determination of six local anesthetics, bupivacaine, lidocaine, mepivacaine, procaine, propanocaine and tetracaine, in pharmaceutical preparations is described. A Spherisorb octadecylsilane ODS-2 C-18 analytical column and spectrophotometric detection at 230 nm were used. The chromatographic behaviour of local anesthetics with different micellar eluents of sodium dodecyl sulphate (SDS) is described. Selection of the adequate composition of the micellar mobile phase (SDS and 1-propanol concentrations) for the analysis of pharmaceuticals was studied. Adequate retention was achieved with an eluent containing 0.15 M SDS +10 % 1-propanol at pH 3. Application of the proposed method to the analysis of eight pharmaceutical formulations gave recoveries between 93 and 100.2 % of the values declared by the manufactures. The proposed procedure for the determination of local anesthetics is rapid, reliable and free from interferences.

引用

页码：85 / 90

页数：6

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