Optimization of enzyme assisted extraction of polysaccharides from Tricholoma matsutake by response surface methodology

Polysaccharides production from the fruiting body of Tricholoma matsutake was carried out using enzyme assisted extraction methodology. Response surface methodology (RSM), based on a five level, four variable central composite design (CCD), was employed to obtain the best possible combination of extraction temperature (X-1: 54-66 degrees C), extraction pH (X-2: 3.6-4.4), extraction time (X-3: 2.4-3.6 h), and complex enzyme (the ratio of papain:pectinase:cellulase was 1:1:1) amount (X-4: 1.6-2.4%) for maximum polysaccharides production. The experimental data obtained were fitted to a second-order polynomial equation using multiple regression analysis and also were analyzed by analysis of variance (ANOVA). The 3-D response surface plot and the contour plot derived from the mathematical models were applied to determine the optimal conditions. The optimum extraction conditions were: extraction temperature of 61.8 degrees C, extraction pH of 4.14, extraction time of 3.2 h, and complex enzyme amount of 2.1% (w/v). Under these conditions, the experimental yield of polysaccharides was 7.53 +/- 0.26%, which is well in close agreement with the value predicted by the model. (C) 2011 Elsevier Ltd. All rights reserved.