Inducible expression and phosphorylation of coactivator BOB.1/OBF.1 in T cells

被引：78

作者：

Zwilling, S

Dieckmann, A

Pfisterer, P

Angel, P

Wirth, T

机构：

[1] UNIV WURZBURG, INST MED STRAHLENKUNDE & ZELLFORSCH, D-97078 WURZBURG, GERMANY

[2] DEUTSCH KREBSFORSCHUNGSZENTRUM, ABT SIGNALTRANSDUKT & WACHSTUMSKONTROLLE, D-69120 HEIDELBERG, GERMANY

来源：

SCIENCE | 1997年 / 277卷 / 5323期

关键词：

D O I：

10.1126/science.277.5323.221

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

BOB.1/OBF.1 is a transcriptional coactivator that is constitutively expressed in B cells and interacts with the Oct1 and Oct2 transcription factors. Upon activation of Jurkat T cells and primary murine thymocytes with phorbol esters and ionomycin, BOB.1/OBF.1 expression and transactivation function were induced. BOB.1/OBF.1 was phosphorylated at Ser(184) both in vivo and in vitro, and this modification was required for inducible activation. Mutation of Ser(184) also diminished transactivation function in B cells, suggesting that the activating phosphorylation that is inducible in T cells is constitutively present in B cells. Thus, BOB.1/OBF.1 is a transcriptional coactivator that is critically regulated by posttranslational modifications to mediate cell type-specific gene expression.

引用

页码：221 / 225

页数：5

共 23 条

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