Cell-dependent gag mutants of HIV-1 are crucially defective at the stage of uncoating/reverse transcription in non-permissive cells

被引：8

作者：

Koh, KB ^{[1
]}

Miyaura, M ^{[1
]}

Yoshida, A ^{[1
]}

Sakurai, A ^{[1
]}

Fujita, M ^{[1
]}

Adachi, A ^{[1
]}

机构：

[1] Univ Tokushima, Sch Med, Dept Virol, Tokushima 7708503, Japan

来源：

MICROBES AND INFECTION | 2000年 / 2卷 / 12期

关键词：

HIV-1; Gag; matrix; capsid; nucleocapsid; reverse transcription;

D O I：

10.1016/S1286-4579(00)01295-8

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

We have previously shown that some of the human immunodeficiency virus type 1 (HIV-1)gag matrix (MA), capsid (CA), and nucleocapsid (NC) mutants display host-cell-dependent replication potential, and that they are defective at the early phase of the virus replication cycle in non-permissive cells. To determine the defective replication stage of the cell-dependent mutants precisely, the processes of virus entry into cells and virus DNA synthesis were monitored by the highly sensitive enzyme-linked immunosorbent assay and polymerase chain reaction amplification analysis. The results obtained indicated that all the cell-dependent MA, CA and NC mutants are defective ar the stage of uncoating/reverse transcription, and that a cellular factor(s) is involved in this process. (C) 2000 Editions scientifiques et medicales Elsevier SAS.

引用

页码：1419 / 1423

页数：5

共 21 条

[1] GENERATION AND CHARACTERIZATION OF THE HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 MUTANTS [J].

ADACHI, A ;

ONO, N ;

SAKAI, H ;

OGAWA, K ;

SHIBATA, R ;

KIYOMASU, T ;

MASUIKE, H ;

UEDA, S .

ARCHIVES OF VIROLOGY, 1991, 117 (1-2) :45-58

[2] PRODUCTION OF ACQUIRED IMMUNODEFICIENCY SYNDROME-ASSOCIATED RETROVIRUS IN HUMAN AND NONHUMAN CELLS TRANSFECTED WITH AN INFECTIOUS MOLECULAR CLONE [J].

ADACHI, A ;

GENDELMAN, HE ;

KOENIG, S ;

FOLKS, T ;

WILLEY, R ;

RABSON, A ;

MARTIN, MA .

JOURNAL OF VIROLOGY, 1986, 59 (02) :284-291

[3]

Adachi Akio, 1996, P43

[4] Cell-dependent requirement of human immunodeficiency virus type 1 gp41 cytoplasmic tail for Env incorporation into virions [J].

Akari, H ;

Fukumori, T ;

Adachi, A .

JOURNAL OF VIROLOGY, 2000, 74 (10) :4891-4893

[5] Nef-induced major histocompatibility complex class I down-regulation is functionally dissociated from its virion incorporation, enhancement of viral infectivity, and CD4 down-regulation [J].

Akari, H ;

Arold, S ;

Fukumori, T ;

Okazaki, T ;

Strebel, K ;

Adachi, A .

JOURNAL OF VIROLOGY, 2000, 74 (06) :2907-2912

[6] Pseudotyping human immunodeficiency virus type 1 by vesicular stomatitis virus G protein does not reduce the cell-dependent requirement of Vif for optimal infectivity: functional difference between Vif and Nef [J].

Akari, H ;

Uchiyama, T ;

Fukumori, T ;

Iida, S ;

Koyama, AH ;

Adachi, A .

JOURNAL OF GENERAL VIROLOGY, 1999, 80 :2945-2949

[7] The HIV-1 Vpr displays strong anti-apoptotic activity [J].

Fukumori, T ;

Akari, H ;

Iida, S ;

Hata, S ;

Kagawa, S ;

Aida, Y ;

Koyama, AH ;

Adachi, A .

FEBS LETTERS, 1998, 432 (1-2) :17-20

[8] HIV-1 capsid mutants inhibit the replication of wild-type virus at both early and late infection phases [J].

Furuta, RA ;

Shimano, R ;

Ogasawara, T ;

Inubushi, R ;

Amano, K ;

Akari, H ;

Hatanaka, M ;

Kawamura, M ;

Adachi, A .

FEBS LETTERS, 1997, 415 (02) :231-234

[9] GAG PROTEINS OF THE HIGHLY REPLICATIVE MN STRAIN OF HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 - POSTTRANSLATIONAL MODIFICATIONS, PROTEOLYTIC PROCESSINGS, AND COMPLETE AMINO-ACID-SEQUENCES [J].

HENDERSON, LE ;

BOWERS, MA ;

SOWDER, RC ;

SERABYN, SA ;

JOHNSON, DG ;

BESS, JW ;

ARTHUR, LO ;

BRYANT, DK ;

FENSELAU, C .

JOURNAL OF VIROLOGY, 1992, 66 (04) :1856-1865

[10] HUMAN-IMMUNODEFICIENCY-VIRUS VPX IS REQUIRED FOR THE EARLY PHASE OF REPLICATION IN PERIPHERAL-BLOOD MONONUCLEAR-CELLS [J].

KAWAMURA, M ;

SAKAI, H ;

ADACHI, A .

MICROBIOLOGY AND IMMUNOLOGY, 1994, 38 (11) :871-878

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