Effect of WAY-100135 on the hippocampal acetylcholine release potentiated by 8-OH-DPAT, a serotonin1A receptor agonist, in normal and p-chlorophenylalanine-treated rats as measured by in vivo microdialysis

被引:29
作者
Nakai, K [1 ]
Fujii, T [1 ]
Fujimoto, K [1 ]
Suzuki, T [1 ]
Kawashima, K [1 ]
机构
[1] Kyoritsu Coll Pharm, Dept Pharmacol, Minato Ku, Tokyo 1058512, Japan
关键词
acetylcholine; hippocampus; 8-hydroxy-2-(di-n-propylamino)tetralin; microdialysis; radioimmunoassay; serotonin(1A) receptor; (+)WAY-100135;
D O I
10.1016/S0168-0102(98)00019-4
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
The mechanisms involved in the enhancement of acetylcholine (ACh) release in the rat hippocampus by 8-hydroxy-2-(di-n-propylamino)tetralin (8-OH-DPAT), a serotonin (5-HT)(1A) receptor agonist, were investigated using in vivo microdialysis. Administration of p-chlorophenylalanine (PCPA, 300 mg/kg, i.p.), a tryptophan hydroxylase inhibitor, 3 days before the dialysis experiments reduced the hippocampal 5-HT content to 30% of that in saline-treated rats, but did not affect basal ACh release in the hippocampus. 8-OH-DPAT administered systemically (0.5 mg/kg, s.c.) or applied locally (30 mu M) into the hippocampus through the dialysis probe significantly enhanced the release of ACh in the hippocampus of PCPA-treated rats to the same degree as that in saline-treated rats. Pretreatment with (+)WAY-100135 (5 mg/kg, i.p.), a selective 5-HT1A receptor antagonist, completely eliminated the enhancement of ACh release induced by locally applied 8-OH-DPAT, but only partially reduced the effects induced by systemically administered 8-OH-DPAT, in both groups of rats. Systemically administered 8-OH-DPAT induced hyperlocomotion in the both saline- and PCPA-treated rats, but this was not eliminated by(+)WAY-100135. 8-OH-DPAT applied locally into the hippocampus did not elicit hyperlocomotion in either group of rats. These results suggest that the modification of endogenous 5-HT release via the 5-HT1A autoreceptor is not involved in the 8-OH-DPAT-induced increase of hippocampal ACh release, and that the increase of ACh release induced by locally applied 8-OH-DPAT involves mainly hippocampal postsynaptic 5-HT1A receptor stimulation. In addition, a possibility that subtypes of 5-HT receptors other than the 5-HT1A receptor, probably 5-HT7 receptor in the septum as well as postsynaptic 5-HT1A receptor in the hippocampus, are involved in the increased hippocampal ACh release induced by systemically administered 8-OH-DPAT is discussed. (C) 1998 Elsevier Science Ireland Ltd. All rights reserved.
引用
收藏
页码:23 / 29
页数:7
相关论文
共 39 条
[1]   REGIONAL CEREBRAL GLUCOSE PHOSPHORYLATION AND BLOOD-FLOW AFTER INSERTION OF A MICRODIALYSIS FIBER THROUGH THE DORSAL HIPPOCAMPUS IN THE RAT [J].
BENVENISTE, H ;
DREJER, J ;
SCHOUSBOE, A ;
DIEMER, NH .
JOURNAL OF NEUROCHEMISTRY, 1987, 49 (03) :729-734
[2]   5-HT1A AGONISTS INCREASE AND 5-HT3 AGONISTS DECREASE ACETYLCHOLINE EFFLUX FROM THE CEREBRAL-CORTEX OF FREELY-MOVING GUINEA-PIGS [J].
BIANCHI, C ;
SINISCALCHI, A ;
BEANI, L .
BRITISH JOURNAL OF PHARMACOLOGY, 1990, 101 (02) :448-452
[3]   EFFECT OF ACUTE MONOAMINE DEPLETION ON 3,4-METHYLENEDIOXYMETHAMPHETAMINE-INDUCED NEUROTOXICITY [J].
BRODKIN, J ;
MALYALA, A ;
NASH, JF .
PHARMACOLOGY BIOCHEMISTRY AND BEHAVIOR, 1993, 45 (03) :647-653
[4]   8-HYDROXY-2-(DI-N-PROPYLAMINO)TETRALIN, A SELECTIVE SEROTONIN1A RECEPTOR AGONIST, REDUCES THE IMMOBILITY OF RATS IN THE FORCED SWIMMING TEST BY ACTING ON THE NUCLEUS RAPHE DORSALIS [J].
CERVO, L ;
GRIGNASCHI, G ;
SAMANIN, R .
EUROPEAN JOURNAL OF PHARMACOLOGY, 1988, 158 (1-2) :53-59
[5]   (S)-N-TERT-BUTYL-3-(4-(2-METHOXYPHENYL)-PIPERAZIN-1-YL)-2-PHENYLPROPANAMIDE [(S)-WAY-100135] - A SELECTIVE ANTAGONIST AT PRESYNAPTIC AND POSTSYNAPTIC 5-HT(1A) RECEPTORS [J].
CLIFFE, IA ;
BRIGHTWELL, CI ;
FLETCHER, A ;
FORSTER, EA ;
MANSELL, HL ;
REILLY, Y ;
ROUTLEDGE, C ;
WHITE, AC .
JOURNAL OF MEDICINAL CHEMISTRY, 1993, 36 (10) :1509-1510
[6]   A critical role for D-1 receptors in the 5-HT1A-mediated facilitation of in vivo acetylcholine release in rat frontal cortex [J].
Consolo, S ;
Ramponi, S ;
Ladinsky, H ;
Baldi, G .
BRAIN RESEARCH, 1996, 707 (02) :320-323
[7]  
CONSOLO S, 1994, J NEUROCHEM, V62, P2254
[8]   CHOLINERGIC MECHANISMS IN DEPRESSION [J].
DILSAVER, SC .
BRAIN RESEARCH REVIEWS, 1986, 11 (03) :285-316
[10]   SILENT 5-HT(1A)-RECEPTOR ANTAGONISTS - UTILITY AS RESEARCH TOOLS AND THERAPEUTIC AGENTS [J].
FLETCHER, A ;
CLIFFE, IA ;
DOURISH, CT .
TRENDS IN PHARMACOLOGICAL SCIENCES, 1993, 14 (12) :441-448