Identification of a novel cell-cycle-induced MCM family protein MCM9

被引:41
作者
Yoshida, K [1 ]
机构
[1] Meiji Univ, Dept Life Sci, Kanagawa 2148571, Japan
关键词
bioinformatics; comparative genomics; MCM; gene regulation; cell cycle;
D O I
10.1016/j.bbrc.2005.03.222
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The minichromosome maintenance (MCM) proteins are essential for DNA replication initiation and elongation in eukaryotes. In mammalian cells, MCM2-MCM7 complexes are believed to unwind DNA during chromosomal DNA replication. Here we identified a novel MCM family gene, MCM9, by using bioinformatics. Human, mouse, and rat MCM9 showed approximately 90-91% total-amino acid identity. MCM9 showed 24-31% total-amino acid identity with MCM2-MCMS protein. Phylogenetic analysis on MCM family members revealed that MCM9 was most closely related to MCM8. Human, mouse, and rat MCM9 gene, consisting of 7, 8, and 7 exons, was mapped to 6q22.1-22.33, 10B3, and 20q11, respectively. We identified transcription factor E2F-binding motifs in the vicinity of the transcription start site among human, mouse, and rat MCM9 gene. MCM9 mRNA was upregulated by transcription factor E2E1 and serum stimulation in NIH3T3 cells. (c) 2005 Elsevier Inc. All rights reserved.
引用
收藏
页码:669 / 674
页数:6
相关论文
共 19 条
[1]   MCM proteins: DNA damage, mutagenesis and repair [J].
Bailis, JM ;
Forsburg, SL .
CURRENT OPINION IN GENETICS & DEVELOPMENT, 2004, 14 (01) :17-21
[2]   DNA replication in eukaryotic cells [J].
Bell, SP ;
Dutta, A .
ANNUAL REVIEW OF BIOCHEMISTRY, 2002, 71 :333-374
[3]  
Freeman A, 1999, CLIN CANCER RES, V5, P2121
[4]   Identification and functional characterization of a new member of the human Mcm protein family:: hMcm8 [J].
Gozuacik, D ;
Chami, M ;
Lagorce, D ;
Faivre, J ;
Murakami, Y ;
Poch, O ;
Biermann, E ;
Knippers, R ;
Bréchot, C ;
Paterlini-Bréchot, P .
NUCLEIC ACIDS RESEARCH, 2003, 31 (02) :570-579
[5]   A DNA helicase activity is associated with an MCM4, -6, and -7 protein complex [J].
Ishimi, Y .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1997, 272 (39) :24508-24513
[6]   A new member of the MCM protein family encoded by the human MCM8 gene, located contrapodal to GCD10 at chromosome band 20p12.3-13 [J].
Johnson, EM ;
Kinoshita, Y ;
Daniel, DC .
NUCLEIC ACIDS RESEARCH, 2003, 31 (11) :2915-2925
[7]   MCM8 is an MCM2-7-related protein that functions as a DNA helicase during replication elongation and not initiation [J].
Maiorano, D ;
Cuvier, O ;
Danis, E ;
Méchali, M .
CELL, 2005, 120 (03) :315-328
[8]   Cell growth-regulated expression of mammalian MCM5 and MCM6 genes mediated by the transcription factor E2F [J].
Ohtani, K ;
Iwanaga, R ;
Nakamura, M ;
Ikeda, M ;
Yabuta, N ;
Tsuruga, H ;
Nojima, H .
ONCOGENE, 1999, 18 (14) :2299-2309
[9]  
Pasion SG, 2001, GENET ENG P, V23, P129
[10]   Sequential MCM/P1 subcomplex assembly is required to form a heterohexamer with replication licensing activity [J].
Prokhorova, TA ;
Blow, JJ .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2000, 275 (04) :2491-2498