Identification of a novel cell-cycle-induced MCM family protein MCM9

被引：42

作者：

Yoshida, K ^{[1
]}

机构：

[1] Meiji Univ, Dept Life Sci, Kanagawa 2148571, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2005年 / 331卷 / 02期

关键词：

bioinformatics; comparative genomics; MCM; gene regulation; cell cycle;

D O I：

10.1016/j.bbrc.2005.03.222

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The minichromosome maintenance (MCM) proteins are essential for DNA replication initiation and elongation in eukaryotes. In mammalian cells, MCM2-MCM7 complexes are believed to unwind DNA during chromosomal DNA replication. Here we identified a novel MCM family gene, MCM9, by using bioinformatics. Human, mouse, and rat MCM9 showed approximately 90-91% total-amino acid identity. MCM9 showed 24-31% total-amino acid identity with MCM2-MCMS protein. Phylogenetic analysis on MCM family members revealed that MCM9 was most closely related to MCM8. Human, mouse, and rat MCM9 gene, consisting of 7, 8, and 7 exons, was mapped to 6q22.1-22.33, 10B3, and 20q11, respectively. We identified transcription factor E2F-binding motifs in the vicinity of the transcription start site among human, mouse, and rat MCM9 gene. MCM9 mRNA was upregulated by transcription factor E2E1 and serum stimulation in NIH3T3 cells. (c) 2005 Elsevier Inc. All rights reserved.

引用

页码：669 / 674

页数：6

共 19 条

[1] MCM proteins: DNA damage, mutagenesis and repair [J].