A protective role for heme oxygenase expression in pancreatic islets exposed to interleukin-1β

Heme oxygenase (KO)-1 expression was investigated in rat isolated pancreatic islets. Freshly isolated islets showed no evidence of HO-1 expression. After a 20-h culture, there was a small increase in HO-I in control islets, and interleukin-lp (IL-lp) induced HO-1 expression above control levels. N-G-monomethyl-L-arginine inhibited the IL-1 beta-induced increase in MO-I. Sodium nitroprusside-generated nitric oxide also increased HO-1 expression. CoCl2 induced a concentration- and time-dependent increase in MO-I, but not heat shock. protein 70, expression. Cobalt chloride (CoCl2) protected islets from the inhibitory effects of IL-lp on glucose-stimulated insulin release and glucose oxidation. Nickel chloride did not mimic the effects of CoCl2. An inhibitor of HO-1 activity, zinc-protoporphyrin IX (ZnPP), prevented the protective effect of CoCl2 on insulin release with IL-1 beta but did not affect HO-1 expression or the inhibitory response to IL-1 beta alone. ZnPP also inhibited the protective effect of hemin in IL-1 beta-treated islets. CoCl2 inhibited the marked increase in islet nitrite production in response to IL-1 beta. Cobalt-protoporphyrin IX (CoPP), which increased HO expression and activity, also protected islets from the inhibitory effects of IL-1 beta, even though IL-1 beta largely blocked the CoPP-induced increase in HO-1 expression. In beta HC9 cells, CoCl2 increased HO-1 expression and HO activity, whereas CoPP directly activated HO. ZnPP inhibited basal and CoCl2-stimulated HO activity. Thus, increased HO-I expression and/or HO activity in response to CoCl2, CoPP, and hemin, seems to mediate protective responses of pancreatic islets against IL-1 beta. HO-1 may be protective of beta-cells because of the scavenging of free heme, the antioxidant effects of the end-product bilirubin, or the generation of carbon monoxide, which might have insulin secretion-promoting effects and inhibitory effects on nitric oxide synthase.