Cloning and nucleotide sequence of the mphB gene for macrolide 2'-phosphotransferase II in Escherichia coli

被引：30

作者：

Noguchi, N

Katayama, J

OHara, K

机构：

[1] Department of Microbiology, School of Pharmacy, Tokyo Univ. of Pharm. and Life S., Hachioji, Tokyo 192-03

[2] Division of Microbial Chemistry, Faculty of Pharmaceutical Science, Chiba University

来源：

FEMS MICROBIOLOGY LETTERS | 1996年 / 144卷 / 2-3期

关键词：

gene cloning; nucleotide sequence; macrolide antibiotic resistance; erythromycin; macrolide 2'-phosphotransferase II; Escherichia coli;

D O I：

10.1016/0378-1097(96)00362-X

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Macrolide 2'-phosphotransferase II [MPH(2')II] inactivates macrolide antibiotics. The mphB gene for MPH(2')II was cloned from Escherichia coli and sequenced. Analysis of the nucleotide sequence indicated that mphB encoded a protein of 302 amino acids with a molecular mass of 34483 Da. The carboxy-terminal region of the deduced protein contained a sequence that resembled a conserved functional domain in aminoglycoside phosphotransferases.

引用

页码：197 / 202

页数：6

共 26 条

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