Enhanced growth of seed viruses for H5N1 influenza vaccines

被引：44

作者：

Horimoto, Taisuke

Murakami, Shin

Muramoto, Yukiko

Yamada, Shinya

Fujii, Ken

Kiso, Maki

Iwatsuki-Horimoto, Kiyoko

Kino, Yolchiro

Kawaoka, Yoshihiro

机构：

[1] Univ Tokyo, Inst Med Sci, Dept Microbiol & Immunol, Div Virol,Minato Ku, Tokyo 108, Japan

[2] Univ Tokyo, Internat Res Ctr Infect Dis, Inst Med Sci, Tokyo 108, Japan

[3] Japan Sci & Technol Agcy, Core Res Evolut Sci & Technol, Saitama 3320012, Japan

[4] Kikuchi Res Ctr, Chemo Sero Therapeut Res Inst, Kumamoto 8691298, Japan

[5] Univ Wisconsin, Sch Vet Med, Dept Pathobiol Sci, Madison, WI 53706 USA

来源：

VIROLOGY | 2007年 / 366卷 / 01期

关键词：

H5N1; influenza; pandemic; vaccine; REVERSE GENETICS; A VIRUSES; GENERATION; VIRULENCE;

D O I：

10.1016/j.virol.2007.07.002

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Seed viruses used to produce inactivated H5N1 influenza vaccines are recombinant viruses with modified avirulent-type hernagglutinin (HA) and intact neuraminidase (NA) genes, both derived from an H5N1 isolate, and all remaining genes from the PR8 strain, which grows well in eggs. However, some reassortants grow suboptimally in eggs, imposing obstacles to timely, cost-efficient vaccine production. Here, we demonstrate that our PR8 strain supports better in ovo growth than the PR8 strain used for the WHO-recommended seed virus, NIBRG-14. Moreover, inclusion of an alternative NA protein further enhanced viral growth in eggs. These findings suggest that our H5N1 vaccine candidates would increase the availability of H5N I vaccine doses at the onset of a new pandemic. (c) 2007 Elsevier Inc. All rights reserved.

引用

页码：23 / 27

页数：5

共 15 条

[1] Improvement of influenza A/Fujian/411/02 (H3N2) virus growth in embryonated chicken eggs by balancing the hemagglutinin and neuraminidase activities, using reverse genetics [J].