Simultaneous determination of illicit drugs in human urine by liquid chromatography mass spectrometry

被引:51
作者
Tatsuno, M
Nishikawa, M
Katagi, M
Tsuchihashi, H
机构
[1] Forensic Science Laboratory, Osaka Pref. Police Headquarters, Chuo-ku, Osaka 541, 1-3-18, Hommachi
关键词
D O I
10.1093/jat/20.5.281
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
The method for simultaneous determination and confirmation of illicit drugs (e.g., methamphetamine, amphetamine, ephedrine, methylephedrine, morphine, morphine-3-glucuronide, morphine-6-glucuronide, 6-acetylmorphine, cocaine, and benzoylecgonine) in human urine by thermospray liquid chromatography-mass spectrometry (LC-MS) was studied. The LC-MS separation was performed on a reversed phase column (L-column ODS; 150 mm x 4.6-mm i.d.) using a gradient mobile phase system of 100mM ammonium acetate for 1 min then linear ramps to 100mM ammonium acetate including 40% acetonitrile at 20 min. Extraction was conducted by solid-phase extraction using a Sep- pak C18 cartridge. The drugs were eluted with 2 mL of 40% acetonitrile in 100mM ammonium acetate, pH3 (adjusted with acetic acid), from the cartridge. A 50-μL volume of the eluate was injected into the LC-MS. The recoveries by this extraction were 88 to 99%. The mass spectra of methamphetamine, amphetamine, ephedrine, methylephedrine, morphine, 6-acetylmorphine, cocaine, and benzoylecgonine showed the quasi-molecular [M+H]+ ion as the base peak, whereas morphine-3-glucuronide and morphine-6-glucuronide showed [MH-glucuronide]+ ion as the base peak. The calibration graphs were linear and reproducible. Detection limits of these drugs ranged from 2 to 40 ng/mL by selected ion monitoring (SIM) mode and from 50 to 400 ng/mL by scan mode. The coefficients of variation for the analysis of these drugs ranged from 4.5 to 9.5% at a concentration of 0.4 μg/mL (n = 10).
引用
收藏
页码:281 / 286
页数:6
相关论文
共 17 条
[1]   URINARY-EXCRETION OF ECGONINE METHYL-ESTER, A MAJOR METABOLITE OF COCAINE IN HUMANS [J].
AMBRE, J ;
FISCHMAN, M ;
RUO, TI .
JOURNAL OF ANALYTICAL TOXICOLOGY, 1984, 8 (01) :23-25
[2]   THE URINARY-EXCRETION OF COCAINE AND METABOLITES IN HUMANS - A KINETIC-ANALYSIS OF PUBLISHED DATA [J].
AMBRE, J .
JOURNAL OF ANALYTICAL TOXICOLOGY, 1985, 9 (06) :241-245
[3]   FORENSIC DRUG-TESTING FOR OPIATES .5. URINE TESTING FOR HEROIN, MORPHINE, AND CODEINE WITH COMMERCIAL OPIATE IMMUNOASSAYS [J].
CONE, EJ ;
DICKERSON, S ;
PAUL, BD ;
MITCHELL, JM .
JOURNAL OF ANALYTICAL TOXICOLOGY, 1993, 17 (03) :156-164
[4]   ASSAY FOR CODEINE, MORPHINE AND 10 POTENTIAL URINARY METABOLITES BY GAS-CHROMATOGRAPHY - MASS FRAGMENTOGRAPHY [J].
CONE, EJ ;
DARWIN, WD ;
BUCHWALD, WF .
JOURNAL OF CHROMATOGRAPHY, 1983, 275 (02) :307-318
[5]  
ELSOHLY MA, 1991, J FORENSIC SCI, V36, P93
[6]   DETECTION OF O-6-MONOACETYLMORPHINE IN URINE SAMPLES BY GC/MS AS EVIDENCE FOR HEROIN USE [J].
FEHN, J ;
MEGGES, G .
JOURNAL OF ANALYTICAL TOXICOLOGY, 1985, 9 (03) :134-138
[8]   QUANTITATION OF METHAMPHETAMINE AND AMPHETAMINE IN URINE BY CAPILLARY GC MS .1. ADVANTAGES OF TRICHLOROACETYL DERIVATIZATION [J].
HORNBECK, CL ;
CZARNY, RJ .
JOURNAL OF ANALYTICAL TOXICOLOGY, 1989, 13 (03) :144-149
[9]   A METHOD FOR THE SIMULTANEOUS DETERMINATION OF COCAINE, BENZOYLECGONINE, AND ECGONINE METHYL-ESTER IN BLOOD AND URINE USING GC EIMS WITH DERIVATIZATION TO PRODUCE HIGH MASS MOLECULAR-IONS [J].
ISENSCHMID, DS ;
LEVINE, BS ;
CAPLAN, YH .
JOURNAL OF ANALYTICAL TOXICOLOGY, 1988, 12 (05) :242-245
[10]   THE QUANTITATIVE CONVERSION OF MORPHINE 3-BETA-D GLUCURONIDE TO MORPHINE USING BETA-GLUCURONIDASE OBTAINED FROM PATELLA-VULGATA AS COMPARED TO ACID-HYDROLYSIS [J].
JENNISON, TA ;
WOZNIAK, E ;
NELSON, G ;
URRY, FM .
JOURNAL OF ANALYTICAL TOXICOLOGY, 1993, 17 (04) :208-210