Histone H4 underacetylation in plant facultative heterochromatin

被引:17
作者
Buzek, J
Riha, K
Siroky, J
Ebert, I
Greilhuber, J
Vyskot, B
机构
[1] Acad Sci Czech Republ, Inst Biophys, CS-61265 Brno, Czech Republic
[2] Univ Vienna, Inst Bot, Dept Systemat Karyol & Embryol, A-1030 Vienna, Austria
关键词
facultative heterochromatin; Gagea lutea pentaploid endosperm; histone H4 acetylation; immunogold labelling; plants; western blot analysis;
D O I
10.1515/bchm.1998.379.10.1235
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
It has been recently shown that facultative heterochromatin in some phyla is H4 and H3 histone underacetylated, Here we present H4 acetylation analyses in a monocotyledonous plant species, Gagea lutea, whose pentaploid endosperm nuclei possess prominent facultative heterochromatin regions, This heterochromatin is attributed to three chromosome sets originated from the chalazal polar nucleus of the embryo sac, We have previously shown that some parts of this heterochromatin contain heavily methylated DNA, but not all the heterochromatin is hypermethylated, In this report we demonstrate that this facultative heterochromatin is characterised by a conspicuous depletion of histone H4 acetylation at N-terminal lysine residues 5, 8, and 12, but not 16, Endosperm metaphases stained with antiserum against H4Ac5 indicated some heavily labelled chromosomes, while the others displayed no signal (presumably those coming from the three heterochromatinised chromosome sets). Western blotting analyses have shown that the antisera used, designed to detect human H4 histones, are suitable to recognise specific isoforms of acetylated H4 histones in plants and that the most abundant H4 in G. lutea leaves occurs in its diacetylated isoform, We conclude that flowering plants, similarly to protozoa, yeasts and animals, evolved core histone acetylation/deacetylation as a long-term transcriptional control mechanism to establish and/or transmit epigenetic information on gene expression.
引用
收藏
页码:1235 / 1241
页数:7
相关论文
共 31 条
  • [1] Belyaev ND, 1996, HUM GENET, V97, P573
  • [2] The acetylation patterns of histones H3 and H4 along Vicia faba chromosomes are different
    Belyaev, ND
    Houben, A
    Baranczewski, P
    Schubert, I
    [J]. CHROMOSOME RESEARCH, 1998, 6 (01) : 59 - 63
  • [3] Braunstein M, 1996, MOL CELL BIOL, V16, P4349
  • [4] Structure and DNA methylation pattern of partially heterochromatinised endosperm nuclei in Gagea lutea (Liliaceae)
    Bu J.
    Ebert I.
    Ruffini-Castiglione M.
    Široký J.
    Vyskot B.
    Greilhuber J.
    [J]. Planta, 1998, 204 (4) : 506 - 514
  • [5] Histones in transit: Cytosolic histone complexes and diacetylation of H4 during nucleosome assembly in human cells
    Chang, L
    Loranger, SS
    Mizzen, C
    Ernst, SG
    Allis, CD
    Annunziato, AT
    [J]. BIOCHEMISTRY, 1997, 36 (03) : 469 - 480
  • [6] GEITLER L, 1950, CHROMOSOMA, V3, P271
  • [7] X-chromosome inactivation in mammals
    Heard, E
    Clerc, P
    Avner, P
    [J]. ANNUAL REVIEW OF GENETICS, 1997, 31 : 571 - 610
  • [8] A DIRECT LINK BETWEEN CORE HISTONE ACETYLATION AND TRANSCRIPTIONALLY ACTIVE CHROMATIN
    HEBBES, TR
    THORNE, AW
    CRANEROBINSON, C
    [J]. EMBO JOURNAL, 1988, 7 (05) : 1395 - 1402
  • [9] Differences of histone H4 acetylation and replication timing between A and B chromosomes of Brachycome dichromosomatica
    Houben, A
    Belyaev, ND
    Leach, CR
    Timmis, JN
    [J]. CHROMOSOME RESEARCH, 1997, 5 (04) : 233 - 237
  • [10] Differential immunostaining of plant chromosomes by antibodies recognizing acetylated histone H4 variants
    Houben, A
    Belyaev, ND
    Turner, BM
    Schubert, I
    [J]. CHROMOSOME RESEARCH, 1996, 4 (03) : 191 - 194