Transplantation of transduced nonhuman primate CD34+ cells using a gibbon ape leukemia virus vector:: restricted expression of the gibbon ape leukemia virus receptor to a subset of CD34+ cells

The transduction efficiencies of immunoselected rhesus macaque (Macaca mulatta) CD34(+) cells and colony-forming progenitor cells based on polymerase chain reaction (PCR) analysis were comparable for an amphotropic Moloney murine leukemia virus (MLV) retroviral vector and a retroviral vector derived from the gibbon ape leukemia virus (GaLV) packaging cell line, PG13. On performing autologous transplantation studies using immunoselected CD34(+) cells transduced with the GaLV envelope (env) retroviral vector, less than 1% of peripheral blood (PB) contained provirus. This was true whether bone marrow (BM) or cytokine-mobilized PB immunoselected CD34(+) cells were reinfused. This level of marking was evident in two animals whose platelet counts never fell below 50 000/mu l and whose leukocyte counts had recovered by days 8 and 10 after having received 1.7 x 10(7) or greater of cytokine-mobilized CD34(+) PB cells/kg. Reverse transcriptase(RT)PCR analysis of CD34(+) subsets for both the GaLV and amphotropic receptor were performed. The expression of the GaLV receptor was determined to be restricted to CD34(+) Thy-1(+) cells, and both CD34(+) CD38(+) and CD34(+) CD38dim cells, while the amphotropic receptor was present on all CD34(+) cell subsets examined. Our findings suggest that, in rhesus macaques, PG13-derived retroviral vectors may only be able to transduce a subset of CD34(+) cells as only CD34(+) Thy-1(+) cells express the GaLV receptor.