A novel cold-inducible gene from Pak-choi (Brassica campestris ssp chinensis), BcWRKY46, enhances the cold, salt and dehydration stress tolerance in transgenic tobacco

被引：79

作者：

Wang, Feng ^{[1
,2
]}

Hou, Xilin ^{[1
,2
]}

Tang, Jun ^{[1
,2
]}

Wang, Zhen ^{[1
,2
]}

Wang, Shuming ^{[1
,2
]}

Jiang, Fangling ^{[1
,2
]}

Li, Ying ^{[1
,2
]}

机构：

[1] Nanjing Agr Univ, State Key Lab Crop Genet & Germplasm Enhancement, Nanjing 210095, Peoples R China

[2] Nanjing Agr Univ, Key Lab So Vegetable Crop Genet Improvement, Minist Agr, Nanjing 210095, Peoples R China

来源：

MOLECULAR BIOLOGY REPORTS | 2012年 / 39卷 / 04期

关键词：

Pak-choi; ABA; Abiotic stress; WRKY; Overexpression; WRKY TRANSCRIPTION FACTOR; DNA-BINDING PROTEINS; SALICYLIC-ACID; ABSCISIC-ACID; EXPRESSION PROFILES; REGULATORY NETWORKS; ARABIDOPSIS; RICE; SUPERFAMILY; RESPONSES;

D O I：

10.1007/s11033-011-1245-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

WRKY TFs belong to one of the largest families of transcriptional regulators in plants and form integral parts of signaling webs that modulate many plant processes. BcWRKY46, a cDNA clone encoding a polypeptide of 284 amino acids and exhibited the structural features of group III of WRKY protein family, was isolated from the cold-treated leaves of Pak-choi (Brassica campestris ssp. chinensis Makino, syn. B. rapa ssp. chinensis) using the cDNA-AFLP technique. Expression of this gene was induced quickly and strongly in response to various environmental stresses, including low temperatures, ABA, salt and dehydration. Constitutive expression of BcWRKY46 in tobacco under the control of the CaMV35S promoter reduced the susceptibility of transgenic tobacco to freezing, ABA, salt and dehydration stresses. Our studies suggest that BcWRKY46 plays an important role in responding to ABA and abiotic stress.

引用

页码：4553 / 4564

页数：12

共 54 条

[1] Transcript imaging with cDNA-AFLP: A step-by-step protocol [J].