Universal COI primers for DNA barcoding amphibians

被引:247
作者
Che, Jing [1 ]
Chen, Hong-Man [1 ,2 ]
Yang, Jun-Xiao [1 ]
Jin, Jie-Qiong [1 ]
Jiang, Ke [1 ]
Yuan, Zhi-Yong [1 ]
Murphy, Robert W. [1 ,3 ]
Zhang, Ya-Ping [1 ,2 ]
机构
[1] Chinese Acad Sci, Kunming Inst Zool, State Key Lab Genet Resources & Evolut, Kunming 650223, Peoples R China
[2] Yunnan Univ, Lab Conservat & Utilizat Bioresources, Kunming 650091, Peoples R China
[3] Royal Ontario Museum, Ctr Biodivers & Conservat Biol, Toronto, ON M5S 2C6, Canada
基金
中国国家自然科学基金; 加拿大自然科学与工程研究理事会;
关键词
Anura; Caudata; forensics; genomics; Gymnophiona; SEQUENCE;
D O I
10.1111/j.1755-0998.2011.03090.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
DNA barcoding is a proven tool for the rapid and unambiguous identification of species, which is essential for many activities including the vouchering tissue samples in the genome 10K initiative, genealogical reconstructions, forensics and biodiversity surveys, among many other applications. A large-scale effort is underway to barcode all amphibian species using the universally sequenced DNA region, a partial fragment of mitochondrial cytochrome oxidase subunit I COI. This fragment is desirable because it appears to be superior to 16S for barcoding, at least for some groups of salamanders. The barcoding of amphibians is essential in part because many species are now endangered. Unfortunately, existing primers for COI often fail to achieve this goal. Herein, we report two new pairs of primers (?, ?) that in combination serve to universally amplify and sequence all three orders of Chinese amphibians as represented by 36 genera. This taxonomic diversity, which includes caecilians, salamanders and frogs, suggests that the new primer pairs will universally amplify COI for the vast majority species of amphibians.
引用
收藏
页码:247 / 258
页数:12
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