The cell surface receptor G6b, a member of the immunoglobulin superfamily, binds heparin

被引：14

作者：

de Vet, ECJM ^{[1
]}

Newland, SAB ^{[1
]}

Lyons, PA ^{[1
]}

Aguado, B ^{[1
]}

Campbell, RD ^{[1
]}

机构：

[1] Rosalind Franklin Ctr Genom Res, MRC, Cambridge CB10 1SB, England

来源：

FEBS LETTERS | 2005年 / 579卷 / 11期

基金：

英国医学研究理事会;

关键词：

immunoglobulin superfamily; heparin; sulfated glycan; MHC;

D O I：

10.1016/j.febslet.2005.03.032

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The G6b gene, located in the human Major Histocompatibility Complex, encodes a receptor of the immunoglobulin (Ig) superfamily. In this study, we show using a variety of techniques that the extracellular domain of the G6b protein, containing a single Ig-like domain, binds to heparin with high affinity. In an ELISA assay, this binding was displaceable with soluble heparin with an IC50 value of approximately 0.5 mu g/ml. Other sulfated glycans showed weaker or no competition. The observed interaction between G6b and heparin is strongly salt dependent suggesting a mainly electrostatic interaction. Heparin might modulate the interaction of G6b with its as yet unidentified protein ligand. (c) 2005 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：2355 / 2358

页数：4

共 19 条

[1] Membrane proteins with immunoglobulin-like domains - a master superfamily of interaction molecules [J].

Barclay, AN .

SEMINARS IN IMMUNOLOGY, 2003, 15 (04) :215-223

[2]

Capila I, 2002, ANGEW CHEM INT EDIT, V41, P391

[3] Structural determinants in the sequences of immunoglobulin variable domain [J].

Chothia, C ;

Gelfand, I ;

Kister, A .

JOURNAL OF MOLECULAR BIOLOGY, 1998, 278 (02) :457-479

[4] Siglecs: sialic-acid-binding immunoglobulin-like lectins in cell-cell interactions and signalling [J].

Crocker, PR .

CURRENT OPINION IN STRUCTURAL BIOLOGY, 2002, 12 (05) :609-615

[5] Adaptor signalling proteins Grb2 and Grb7 are recruited by human G6f, a novel member of the immunoglobulin superfamily encoded in the MHC [J].

de Vet, ECJM ;

Aguado, B ;

Campbell, RD .

BIOCHEMICAL JOURNAL, 2003, 375 :207-213

[6] G6b, a novel immunoglobulin superfamily member encoded in the human major histocompatibility complex, interacts with SHP-1 and SHP-2 [J].

de Vet, ECJM ;

Aguado, B ;

Campbell, RD .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2001, 276 (45) :42070-42076

[7] DIFFERENCES IN THE INTERACTION OF HEPARIN WITH ARGININE AND LYSINE AND THE IMPORTANCE OF THESE BASIC-AMINO-ACIDS IN THE BINDING OF HEPARIN TO ACIDIC FIBROBLAST GROWTH-FACTOR [J].

FROMM, JR ;

HILEMAN, RE ;

CALDWELL, EEO ;

WEILER, JM ;

LINHARDT, RJ .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1995, 323 (02) :279-287

[8]

Hasan M, 1999, J IMMUNOL, V162, P1064

[9] Polyanions and the proteome [J].

Jones, LS ;

Yazzie, B ;

Middaugh, CR .

MOLECULAR & CELLULAR PROTEOMICS, 2004, 3 (08) :746-769

[10] CLEAVAGE OF STRUCTURAL PROTEINS DURING ASSEMBLY OF HEAD OF BACTERIOPHAGE-T4 [J].

LAEMMLI, UK .

NATURE, 1970, 227 (5259) :680-+

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