Sensitive and specific detection of staphylococcal epidermolysins A and B in broth cultures by flow cytometry-assisted multiplex immunoassay

被引：5

作者：

Joubert, O ^{[1
]}

Keller, D ^{[1
]}

Pinck, A ^{[1
]}

Monteil, H ^{[1
]}

Prévost, G ^{[1
]}

机构：

[1] Fac Med, Inst Bacteriol, Lab Physiopathol Antibiol Infect Nosocomiales & E, UPRES EA3432, F-67000 Strasbourg, France

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2005年 / 43卷 / 03期

关键词：

D O I：

10.1128/JCM.43.3.1076-1080.2005

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Two of the most common bacterial skin infections of young infants and children are bullous impetigo due to Staphylococcus aureus and its more acute form, staphylococcal scalded skin syndrome. Epidermolysin A (ETA), ETB and, possibly, ETD are responsible for these diseases, which may appear as epidemics in pediatric patients. We tested the reliability of a flow cytometry-assisted multiplex immunoassay (Bio-Plex system) for the detection of ETA and ETB. The Bio-Plex system was found to be highly specific and highly sensitive for toxin concentrations of between 2 and 80,000 pg/ml. The results of this assay were 100% identical to the results of a PCR-based method. We demonstrated that this test did not generate any cross-reactions with ETD-producing isolates. The level of detection of ETB by this test differed according to culture conditions and from isolate to isolate; these results must be taken into account for diagnostic purposes.

引用

页码：1076 / 1080

页数：5

共 32 条

[1] Staphylococcal exfoliative toxin B specifically cleaves desmoglein 1 [J].