A rapid and sensitive method for the analysis of cyanophycin

被引：13

作者：

Erickson, NA

Kolodny, NH

Allen, MM ^{[1
]}

机构：

[1] Wellesley Coll, Dept Biol Sci, Wellesley, MA 02481 USA

[2] Wellesley Coll, Dept Chem, Wellesley, MA 02481 USA

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS | 2001年 / 1526卷 / 01期

基金：

美国国家科学基金会;

关键词：

cyanobacterium; cyanophycin; nitrogen reserve; H-1 nuclear magnetic resonance spectroscopy; arginine analysis;

D O I：

10.1016/S0304-4165(01)00098-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A method has been devised for the quantitative analysis of cyanophycin, based on H-1 nuclear magnetic resonance (NMR) spectroscopy, allowing determination of the nitrogen status of cyanobacteria. Cyanophycin is extracted with minimal washing from small volumes of cells and quantified by integration of the NMR peak attributed to the protons attached to the delta -carbon of arginine. Linear relationships were found between the amount of cyanophycin determined by this method and both known concentrations of cyanophycin solutions and the amount of cyanophycin determined using the standard chemical arginine assay. (C) 2001 Elsevier Science B.V. All rights reserved.

引用

页码：5 / 9

页数：5

共 12 条

[1] Molecular characterization of the cyanophycin synthetase from Synechocystis sp strain PCC6308 [J].