PVA and BSA stabilized silver nanoparticles based surface-enhanced plasmon resonance probes for protein detection

被引：64

作者：

Ananth, A. Nimrodh ^{[2
]}

Daniel, S. C. G. Kiruba ^{[1
]}

Sironmani, T. Anitha ^{[1
]}

Umapathi, S. ^{[2
]}

机构：

[1] Madurai Kamaraj Univ, Sch Biotechnol, Madurai 625021, Tamil Nadu, India

[2] Madurai Kamaraj Univ, Sch Phys, Madurai 625021, Tamil Nadu, India

来源：

COLLOIDS AND SURFACES B-BIOINTERFACES | 2011年 / 85卷 / 02期

关键词：

Silver nanoparticles; PVA/BSA stabilization of nanoparticles; Biocompatibility study; Mice; Biosensor; IRON-OXIDE NANOPARTICLES; GOLD NANOPARTICLES; POLYVINYL-ALCOHOL; BIOSENSORS; NANOCRYSTALS; MONOLAYERS; PEPTIDES; MATRIX; AGENT; SIZE;

D O I：

10.1016/j.colsurfb.2011.02.012

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

To perform biosensing using nanoparticles in solution, silver particles were coated with bovine serum albumin (BSA) and polyvinyl alcohol (PVA) as control stabilizer. The plasmon resonance (420 nm) of the silver nanoparticles in solution was shifted slightly to longer wavelength (443 nm) when they were coated with BSA. The biointeractions of these engineered nanoparticles were studied using a mouse model. No significant changes in behavior or toxicity were observed. The nanoparticles were detected in all tissues including the brain. Antibody recognition was monitored via the change in light absorption which accompanied binding, indicating that the particles can be used as a biosensor to gain more insight into cellular mechanisms governing the function of organs in general, and the blood brain barrier (BBB) and brain in particular. (C) 2011 Elsevier B.V. All rights reserved.

引用

页码：138 / 144

页数：7

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