Objectives: No widely used triage instrument accurately assesses patient acuity. The Emergency Severity Index (EST) promises to facilitate reliable acuity assessment and possibly predict patient disposition. However, reliability and validity of EST scores have not been established in emergency departments (EDs) outside the original research sites, and version 3 (v.3) of the EST has not been evaluated. The study hypothesis was that scores on the EST v.3 show good interrater reliability and predict hospital admission, admission site, and death. Methods: The authors conducted an ED-based cross-sectional retrospective study of 403 systematically selected ED records of patients who presented to an academic medical center. Twenty-seven variables were abstracted, including triage level assigned, admission status, site, and death. Using a standard process, the researchers determined the true triage level. Weighted kappa and Pearson correlation were used to calculate interrater reliability between true triage level and triage score assigned by the registered nurse (RN). The relationships between the true EST level and admission, admission site, and death were assessed. Results: Interrater reliability between RN EST level and the true EST level was kappa = 0.89; Pearson r = 0.83 (p < 0.001). Hospital admission by EST level was as follows: 1 (80%), 2 (73%), 3 (51%), 4 (6%), and 5 (5%). A higher percentage of EST level-1 and level-2 patients (40%, 12%) were admitted to the intensive care unit than EST levels 3-5 (2%, 0%, 0%). Admission to telemetry for EST levels 1-5 was 20%, 19%, 7%, 1%, and 0%, respectively. Three of four patients who died were EST level 1 or 2. Conclusions: Scores on the EST assigned by nurses have excellent interrater reliability and predict hospital admission and location of admission.