Correlation between viral resistance to zidovudine and resistance at the reverse transcriptase level for a panel of human immunodeficiency virus type 1 mutants

被引：45

作者：

Lennerstrand, J

Hertogs, K

Stammers, DK

Larder, BA

机构：

[1] Virco UK Ltd, Cambridge CB4 0GA, England

[2] Univ Oxford, Struct Biol Div, Wellcome Trust Ctr Human Genet, Oxford, England

[3] Virco NV, B-2800 Mechelen, Belgium

来源：

JOURNAL OF VIROLOGY | 2001年 / 75卷 / 15期

关键词：

D O I：

10.1128/JVI.75.15.7202-7205.2001

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Using a large panel of human immunodeficiency virus type 1 site-directed mutants, we have observed a higher correlation than has previously been demonstrated between zidovudine (AZT)-triphosphate resistance data at the reverse transcriptase (RT) level and corresponding viral AZT resistance. This enhanced-resistance effect at the RT level was seen with ATP and to a lesser extent with PP, when ATP was added at physiological concentrations. The ATP-dependent mechanism (analogous to pyrophosphorolysis) appears to be dominant in the mutants bearing the D67N and K70R or 69 insertion mutations, whereas the Q151M mutation seems independent of ATP for decreased binding to AZT-triphosphate.

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页码：7202 / 7205

页数：4

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