Saturation-transfer difference NMR studies for the epitope mapping of a carbohydrate-mimetic peptide recognized by an anti-carbohydrate antibody

被引：30

作者：

Johnson, MA

Pinto, BM ^{[1
]}

机构：

[1] Simon Fraser Univ, Dept Chem, Burnaby, BC V5A 1S6, Canada

[2] Simon Fraser Univ, Dept Mol Biol & Biochem, Burnaby, BC V5A 1S6, Canada

来源：

BIOORGANIC & MEDICINAL CHEMISTRY | 2004年 / 12卷 / 01期

基金：

加拿大自然科学与工程研究理事会;

关键词：

peptide-carbohydrate mimicry; saturation-transfer difference NMR; peptide epitope mapping; molecular recognition in solution vs. solid state;

D O I：

10.1016/j.bmc.2003.09.041

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Saturation-transfer difference NMR spectroscopy (STD-NMR) experiments have been performed to analyze the topography or epitope of the octapeptide MDWNMHAA recognized by the anti-carbohydrate antibody SYA/J6 in solution; the antibody is directed against the Shigella flexneri Y O-antigen polysaccharide. The results permit a valuable comparison of solution versus crystal-structure data, and provide insight for the design of the next-generation binding ligands. (C) 2003 Elsevier Ltd. All rights reserved.

引用

页码：295 / 300

页数：6

共 36 条

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