Differential modulation of mu- and delta-opioid antinociception by neuropeptide FF receptors in young mice

The ability of neuropeptide FF (NPFF) to modulate mu- and delta-opioid-induced analgesia by intracerebroventricular administration was compared in adults and 14-day-old mice. In adults, opioid-induced analgesia was predominantly mediated by mu-receptors whereas mu- and delta-receptors were equally involved in pups. An NPFF analog, 1DMe, reduced the analgesic effect of DAGO and [D.Ala(2)]deltorphin-I, mu and delta selective agonists respectively. However, a high dose of 1DMe (22 nmol) increased both morphine and [D.Ala(2)]deltorphin-I-induced analgesia. Dose-response curves for 1DMe in the presence of naltrindole or naltrexone, delta- and mu-opioid selective antagonists respectively, indicate that 1DMe preferentially reversed mu-receptor-mediated but increased delta-receptor-mediated analgesia. These findings demonstrate differences in control of mu- and delta-induced analgesia by NPFF receptors.