Chemical characterization and immunostimulatory effects of a polysaccharide from Polygoni Multiflori Radix Praeparata in cyclophosphamide-induced anemic mice

被引：62

作者：

Chen, Qin ^{[2
]}

Zhang, Song-zhao ^{[2
]}

Ying, Hua-zhong ^{[1
]}

Dai, Xiao-yan ^{[3
]}

Li, Xiao-xian ^{[3
]}

Yu, Chen-huan ^{[1
]}

Ye, Hai-chao ^{[3
]}

机构：

[1] Zhejiang Acad Med Sci, Zhejiang Key Lab Expt Anim, Hangzhou 310013, Zhejiang, Peoples R China

[2] Zhejiang Univ, Coll Med, Affiliated Hosp 2, Dept Clin Lab Med, Hangzhou 310009, Zhejiang, Peoples R China

[3] Zhejiang Chinese Med Univ, Coll Pharmaceut Sci, Hangzhou 310053, Zhejiang, Peoples R China

来源：

CARBOHYDRATE POLYMERS | 2012年 / 88卷 / 04期

关键词：

Polysaccharides; Characterization; Immunomodulation; Antioxidant activity; Anti-anemia; Erythropoietin; RESPONSE-SURFACE METHODOLOGY; EPO RECEPTOR; EXTRACTION; OPTIMIZATION; CONSTITUENTS; BCL-X(L);

D O I：

10.1016/j.carbpol.2012.02.055

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

A major polysaccharide fraction (PMPP) was obtained by purifying the crude polysaccharides extracted from Polygoni Multiflori Radix Praeparata. Preliminary structural characterization was conducted by physicochemical property and Fourier transform infrared spectroscopy. Monosaccharide component analysis indicated that PMPP was composed of rhamnose, arabinose, xylose and glucose in a molar ratio of 1.64:1.00:1.34:6.06. Intraperitoneal administration of PMPP could increase levels of serum IL-2 and hematological parameters (red blood cells, white blood cells and platelet counts), enhance antioxidant profiles, and promote hematopoiesis of splenocytes though up-regulating expressions of EPOR and GATA-1 proteins in cyclophosphamide (Cy) induced anemic mice. These findings indicate that PMPP played an important role in the protection against myelosuppression and immunosuppression in Cy-treated mice and may warrant further evaluation as a potential immunomodulatory agent. (C) 2012 Elsevier Ltd. All rights reserved.

引用

页码：1476 / 1482

页数：7

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