Genetic diversity of rhizobia isolated from Astragalus adsurgens growing in different geographical regions of China

被引：65

作者：

Gao, JL

Terefework, ZD

Chen, WX

Lindström, K

机构：

[1] China Agr Univ, Coll Biol Sci, Dept Microbiol, Beijing 100094, Peoples R China

[2] Univ Helsinki, Bioctr 1, Dept Appl Chem & Microbiol, FIN-00014 Helsinki, Finland

来源：

JOURNAL OF BIOTECHNOLOGY | 2001年 / 91卷 / 2-3期

关键词：

Astragalus adsurgens; rhizobia; diversity; PCR fingerprinting; phylogeny;

D O I：

10.1016/S0168-1656(01)00337-6

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The genetic diversity among 95 isolates from Astragalus adsurgens was investigated using molecular biological methods. All of the isolates and 24 reference strains could be differentiated by AFLP, REP-, ERIC- and BOX-PCR fingerprinting analysis. By cluster analysis of the data, 31 AFLP and 38 Rep-PCR genomic groups were delineated, indicating considerable genetic diversity among the isolates. Fifty-four representative strains were further analyzed by RFLP of PCR-amplified 16S and 23S rDNA, revealing 26 rDNA genotypes among the isolates. The phylogenetic relationship of the isolates was. determined by partial sequencing of 16S rRNA genes of 16 strains. The results suggest that the A. adsurgens rhizobia belong to the genera Agrobacterium, Mesorhizobium, Rhizobium and Sinorhizobium. (C) 2001 Elsevier Science B.V. All rights reserved.

引用

页码：155 / 168

页数：14

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