Epstein-Barr virus infection in vitro can rescue germinal center B cells with inactivated immunoglobulin genes
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作者:
Chaganti, S
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机构:Univ Birmingham, Canc Res UK Inst Canc Studies, Birmingham B15 2TT, W Midlands, England
Chaganti, S
Bell, AI
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机构:Univ Birmingham, Canc Res UK Inst Canc Studies, Birmingham B15 2TT, W Midlands, England
Bell, AI
Pastor, NB
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机构:Univ Birmingham, Canc Res UK Inst Canc Studies, Birmingham B15 2TT, W Midlands, England
Pastor, NB
Milner, AE
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机构:Univ Birmingham, Canc Res UK Inst Canc Studies, Birmingham B15 2TT, W Midlands, England
Milner, AE
Drayson, M
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机构:Univ Birmingham, Canc Res UK Inst Canc Studies, Birmingham B15 2TT, W Midlands, England
Drayson, M
Gordon, J
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机构:Univ Birmingham, Canc Res UK Inst Canc Studies, Birmingham B15 2TT, W Midlands, England
Gordon, J
Rickinson, AB
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Univ Birmingham, Canc Res UK Inst Canc Studies, Birmingham B15 2TT, W Midlands, EnglandUniv Birmingham, Canc Res UK Inst Canc Studies, Birmingham B15 2TT, W Midlands, England
Rickinson, AB
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[1] Univ Birmingham, Canc Res UK Inst Canc Studies, Birmingham B15 2TT, W Midlands, England
[2] Univ Birmingham, MRC, Ctr Immune Regulat, Birmingham B15 2TT, W Midlands, England
Immunoglobulin genotyping of Epstein-Barr virus (EBV)-positive posttransplantation lymphoproliferative disease has suggested that such lesions often arise from atypical post-germinal center B cells, in some cases carrying functionally inactivated immunoglobulin genes. To investigate whether EBV can rescue cells that are failed products of the somatic hypermutation process occurring in germinal centers (GCs), we isolated GC cells from tonsillar cell suspensions and exposed them to EBV in vitro. Screening more than 100 EBV-transformed cell lines of GC origin identified 6 lines lacking surface immunoglobulin, a phenotype never seen among lines derived from circulating naive or memory B cells. Furthermore, 3 of the 6 surface immunoglobulin-negative GC lines carried inactivating mutations in the immunoglobulin H (IgH) variable gene sequence. The ability of EBV to rescue aberrant products of the germinal center reaction in vitro strengthens the probability that a parallel activity contributes to EBV's lymphomagenic potential in vivo.
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Univ Birmingham, Canc Res UK Inst Canc Studies, Birmingham, W Midlands, EnglandUniv Birmingham, Canc Res UK Inst Canc Studies, Birmingham, W Midlands, England
Kelly, G
Bell, A
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Univ Birmingham, Canc Res UK Inst Canc Studies, Birmingham, W Midlands, EnglandUniv Birmingham, Canc Res UK Inst Canc Studies, Birmingham, W Midlands, England
Bell, A
Rickinson, A
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Univ Birmingham, Canc Res UK Inst Canc Studies, Birmingham, W Midlands, EnglandUniv Birmingham, Canc Res UK Inst Canc Studies, Birmingham, W Midlands, England
机构:
Univ Birmingham, Canc Res UK Inst Canc Studies, Birmingham, W Midlands, EnglandUniv Birmingham, Canc Res UK Inst Canc Studies, Birmingham, W Midlands, England
Kelly, G
Bell, A
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Univ Birmingham, Canc Res UK Inst Canc Studies, Birmingham, W Midlands, EnglandUniv Birmingham, Canc Res UK Inst Canc Studies, Birmingham, W Midlands, England
Bell, A
Rickinson, A
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Univ Birmingham, Canc Res UK Inst Canc Studies, Birmingham, W Midlands, EnglandUniv Birmingham, Canc Res UK Inst Canc Studies, Birmingham, W Midlands, England