UORFs, reinitiation and alternative translation start sites in human mRNAs

被引：52

作者：

Kochetov, Alex V. ^{[1
,2
]}

Ahmad, Shandar ^{[3
]}

Ivanisenko, Vladimir ^{[1
]}

Volkova, Oxana A. ^{[1
]}

Kolchanov, Nikolay A. ^{[1
,2
]}

Sarai, Akinori ^{[4
]}

机构：

[1] Russian Acad Sci, Inst Cytol & Genet, Novosibirsk 630090, Russia

[2] Novosibirsk State Univ, Novosibirsk 630090, Russia

[3] Natl Inst Biomed Innovat, Ibaraki, Osaka 5670085, Japan

[4] Kyushu Inst Technol, Iizuka, Fukuoka 8208502, Japan

来源：

FEBS LETTERS | 2008年 / 582卷 / 09期

基金：

日本学术振兴会; 俄罗斯基础研究基金会;

关键词：

eukaryotic mRNA; reinitiation; upstream ORF; protein isoforms; translational control;

D O I：

10.1016/j.febslet.2008.03.014

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

It is known that eukaryotic ribosomes are able to translate small ORFs and reinitiate translation at downstream start codons. However, this mechanism is widely considered to be inefficient and it is not commonly taken into account. We compiled a sample of human mRNAs containing small upstream ORFs overlapping with annotated protein coding sequences. Statistical analysis supported the hypothesis on reinitiation of translation at downstream AUG codons and functional significance of potential alternative ORFs. It may be assumed that some 50UTR- located upstream ORFs can deliver ribosomes to alternative translation starts, and they should be taken into consideration in the prediction of human mRNA coding potential. (C) 2008 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.

引用

页码：1293 / 1297

页数：5

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