Palmitoylated calnexin is a key component of the ribosome-translocon complex

被引:133
作者
Lakkaraju, Asvin K. K. [1 ]
Abrami, Laurence [1 ]
Lemmin, Thomas [2 ]
Blaskovic, Sanja [1 ]
Kunz, Beatrice [1 ]
Kihara, Akio [3 ]
Dal Peraro, Matteo [2 ]
van der Goot, Francoise Gisou [1 ]
机构
[1] Ecole Polytech Fed Lausanne, Gobal Hlth Inst, CH-1015 Lausanne, Switzerland
[2] Ecole Polytech Fed Lausanne, Inst Bioengn, CH-1015 Lausanne, Switzerland
[3] Hokkaido Univ, Fac Pharmaceut Sci, Sapporo, Hokkaido 060, Japan
基金
瑞士国家科学基金会;
关键词
calnexin; DHHC6; endoplasmic reticulum folding; palmitoylation; MOLECULAR-DYNAMICS; PROTEIN; LOCALIZATION; GLYCOPROTEINS; DOMAIN;
D O I
10.1038/emboj.2012.15
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A third of the human genome encodes N-glycosylated proteins. These are co-translationally translocated into the lumen/membrane of the endoplasmic reticulum (ER) where they fold and assemble before they are transported to their final destination. Here, we show that calnexin, a major ER chaperone involved in glycoprotein folding is palmitoylated and that this modification is mediated by the ER palmitoyltransferase DHHC6. This modification leads to the preferential localization of calnexin to the perinuclear rough ER, at the expense of ER tubules. Moreover, palmitoylation mediates the association of calnexin with the ribosome-translocon complex (RTC) leading to the formation of a supercomplex that recruits the actin cytoskeleton, leading to further stabilization of the assembly. When formation of the calnexin-RTC supercomplex was affected by DHHC6 silencing, mutation of calnexin palmitoylation sites or actin depolymerization, folding of glycoproteins was impaired. Our findings thus show that calnexin is a stable component of the RTC in a manner that is exquisitely dependent on its palmitoylation status. This association is essential for the chaperone to capture its client proteins as they emerge from the translocon, acquire their N-linked glycans and initiate folding. The EMBO Journal (2012) 31, 1823-1835. doi: 10.1038/emboj.2012.15; Published online 7 February 2012
引用
收藏
页码:1823 / 1835
页数:13
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