Molecular and phenotypic characterization of strains nodulating Anthyllis vulneraria in mine tailings, and proposal of Aminobacter anthyllidis sp nov., the first definition of Aminobacter as legume-nodulating bacteria

被引：39

作者：

Maynaud, Geraldine ^{[2
]}

Willems, Anne ^{[3
]}

Soussou, Souhir ^{[1
]}

Vidal, Celine ^{[2
]}

Maure, Lucette ^{[2
]}

Moulin, Lionel ^{[4
]}

Cleyet-Marel, Jean-Claude ^{[2
]}

Brunel, Brigitte ^{[1
]}

机构：

[1] Univ Montpellier 2, Montpellier SupAgro, Lab Symbioses Trop & Mediterraneennes, IRD INRA CIRAD Montpellier SupAgro, F-34398 Montpellier 5, France

[2] Univ Montpellier 2, INRA, Lab Symbioses Trop & Mediterraneennes, IRD INRA CIRAD Montpellier SupAgro,USC1242, F-34398 Montpellier 5, France

[3] Univ Ghent, Microbiol Lab, Fac Wetenschappen, Ghent, Belgium

[4] Univ Montpellier 2, IRD, Lab Symbioses Trop & Mediterraneennes, IRD INRA CIRAD Montpellier SupAgro, F-34398 Montpellier 5, France

来源：

SYSTEMATIC AND APPLIED MICROBIOLOGY | 2012年 / 35卷 / 02期

关键词：

Mesorhizobium; Heavy-metal tolerance; Multilocus sequence analysis; nodA gene; N-2-fixation; RHIZOBIUM-LOTI; MESORHIZOBIUM; DIVERSITY; CICERI;

D O I：

10.1016/j.syapm.2011.11.002

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Bacterial strains from Zn-Pb mine tailings were isolated by trapping with Anthyllis vulneraria, a legume-host suitable for mine substratum phytostabilisation. Sequence analysis of the 16S rRNA gene and three housekeeping genes (atpD, dnaK and recA) showed that they were related to those of the genus Aminobacter. DNA-DNA relatedness of representative isolates supported the placement of novel strains in Aminobacter as a new species. Phenotypic data emphasize their differentiation from the other related species of Aminobacter and Mesorhizobium. Aminobacter isolates exhibited nodA sequences tightly related with M. loti as the closest nodA relative. By contrast, their nodA sequences were highly divergent from those of M. metallidurans, another species associated with A. vulneraria that carries two complete copies of nodA. Therefore, the novel bacterial strains efficient on A. vulneraria represented the first occurrence of legume symbionts in the genus Aminobacter. They represent a new species for which the name Aminobacter anthyllidis sp. nov. is proposed (type strain STM4645(T) = LMG26462(T) = CFBP7437(T)). (C) 2011 Elsevier GmbH. All rights reserved.

引用

页码：65 / 72

页数：8

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