Identification and characterization of simple sequence repeat markers from Brassica napus expressed sequences

被引：19

作者：

Batley, Jacqueline ^{[1
]}

Hopkins, Clare J.

Cogan, Noel O. I.

Hand, Melanie

Jewell, Erica

Kaur, Jatinder

Kaur, Sukhjiwan

Li, Xi

Ling, Alison E.

Love, Christopher

Mountford, Hayley

Todorovic, Marija

Vardy, Megan

Walkiewicz, Marzena

Spangenberg, German C.

Edwards, David

机构：

[1] Univ Queensland, Sch Land Crop & Food Sci, Australian Ctr Plant Funct Genom, Brisbane, Qld 4072, Australia

[2] La Trobe R&D Pk, Victorian AgriBiosci Ctr, Dept Primary Ind, Ctr Plant Biotechnol, Bundoora, Vic 3083, Australia

来源：

MOLECULAR ECOLOGY NOTES | 2007年 / 7卷 / 05期

关键词：

Brassica; canola; cross-amplification; EST; genome specificity; simple sequence repeats;

D O I：

10.1111/j.1471-8286.2007.01738.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The availability of expressed sequence data derived from gene discovery programs enables mining for simple sequence repeats (SSR), providing useful genetic markers for crop improvement. These markers are inexpensive, require minimal labour to produce and can frequently be associated with functionally annotated genes. This study presents the development and characterization of 24 expressed sequence tags (EST)-SSR markers from Brassica napus and their cross-amplification across Brassica species. The markers show reliable amplification, genome specificity and considerable polymorphism, demonstrating the utility of EST-SSRs for genetic analysis of wild Brassica populations and commercial Brassica germplasm.

引用

页码：886 / 889

页数：4

共 5 条

[1]

Edwards KJ, 1996, BIOTECHNIQUES, V20, P758

[2] SSRPrimer and SSR Taxonomy Tree:: Biome SSR discovery [J].

Jewell, Erica ;

Robinson, Andrew ;

Savage, David ;

Erwin, Tim ;

Love, Christopher G. ;

Lim, Geraldine A. C. ;

Li, Xi ;

Batley, Jacqueline ;

Spangenberg, German C. ;

Edwards, David .

NUCLEIC ACIDS RESEARCH, 2006, 34 :W656-W659

[3] Integrating and interrogating diverse Brassica data within an EnsEMBL structured database [J].

Love, Christopher ;

Logan, Erica ;

Erwin, Tim ;

Kaur, Jatinder ;

Lim, Geraldine A. C. ;

Hopkins, Clare ;

Batley, Jacqueline ;

James, Nick ;

May, Sean ;

Spangenberg, German ;

Edwards, David .

PROCEEDINGS OF THE JOINT MEETING OF THE FOURTEENTH CRUCIFER GENETICS WORKSHOP AND FOURTH ISHS SYMPOSIUM ON BRASSICAS, 2006, (706) :77-+

[4] Simple sequence repeat marker loci discovery using SSR primer [J].

Robinson, AJ ;

Love, CG ;

Batley, J ;

Barker, G ;

Edwards, D .

BIOINFORMATICS, 2004, 20 (09) :1475-1476

[5]

U. N., 1935, Japanese Journal of Botany, V7, P389

← 1 →