A disaccharide repeat unit is the major structure in fucoidans from two species of brown algae

被引：227

作者：

Chevolot, L

Mulloy, B

Ratiskol, J

Foucault, A

Colliec-Jouault, S

机构：

[1] Univ Paris 13, Lab Rech Macromol, Unite Rech Marine 2, F-93430 Villetaneuse, France

[2] Univ Paris 13, Unite Mixte Rech, CNRS 7540, F-93430 Villetaneuse, France

[3] IFREMER, Unite Rech Marine 2, VP BM, F-44311 Nantes, France

[4] IFREMER, Lab Biochim & Mol Marines, VP BM, F-44311 Nantes, France

[5] Natl Inst Biol Stand & Controls, Mol Struct Lab, Potters Bar EN6 3QG, Herts, England

来源：

CARBOHYDRATE RESEARCH | 2001年 / 330卷 / 04期

关键词：

fucoidan; fucan structures; fucan NMR; brown algae;

D O I：

10.1016/S0008-6215(00)00314-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The predominant repeating structure of a fraction of the fucoidan from Ascophyllum nodosum prepared by acid hydrolysis and centrifugal partition chromatography (LMWF) was established as: [ --> 3)-alpha -L-Fuc(2SO(3)(-))-(1 --> 4)-alpha -L-Fuc(2,3diSO(3)(-))-(1](n) by NMR spectroscopy and methylation analysis. The proton and carbon NMR spectra of this unit have been assigned and found to correspond with features in the spectra of the whole purified fucan from A. nodosum which account for most of the integrated intensity. The same structure has also been recognised in the fucoidan of Fucus vesiculosus. The fraction LMWF has in vitro anticoagulant activity, indicating that the above structure may be partly responsible for biological activity in the native fucoidan. (C) 2001 Elsevier Science Ltd. All rights reserved.

引用

页码：529 / 535

页数：7

共 19 条

[1] Sulfated polysaccharides from the egg jelly layer are species-specific inducers of acrosomal reaction in sperms of sea urchins [J].