Reduced skeletal muscle calpain-10 transcript level is due to a cumulative decrease in major isoforms

被引:29
作者
Yang, XL
Pratley, RE
Baier, LJ
Horikawa, Y
Bell, GI
Bogardus, C
Permana, PA
机构
[1] NIDDKD, Clin Diabet & Nutr Sect, Pheonix Epidemiol & Clin Res Branch, NIH, Phoenix, AZ 85016 USA
[2] Univ Chicago, Howard Hughes Med Inst, Chicago, IL 60637 USA
[3] Univ Chicago, Dept Biochem & Mol Biol, Chicago, IL 60637 USA
[4] Univ Chicago, Dept Med & Human Genet, Chicago, IL 60637 USA
关键词
type; 2; diabetes; Pima Indians; Calpain-10; isoforms; skeletal muscle;
D O I
10.1006/mgme.2001.3171
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
The DNA polymorphism SNP-43 in the calpain-10 gene is associated with insulin resistance and reduced skeletal muscle transcript in Pima Indians. Alternative splicing generates transcript isoforms calpain-10a to -10h. We determined the contribution of calpain-10 mRNA isoforms to the decreased total skeletal muscle calpain-10 mRNA levels observed in the G/G homozygotes. The expression levels of the major isoforms, calpain-10a and -10f, were positively correlated with the total calpain-10 mRNA levels, indicating a cumulative effect. (C) 2001 Academic Press.
引用
收藏
页码:111 / 113
页数:3
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