Multiplex PCR for direct detection of Shiga toxigenic Escherichia coli strains producing the novel subtilase cytotoxin

被引：88

作者：

Paton, AW ^{[1
]}

Paton, JC ^{[1
]}

机构：

[1] Univ Adelaide, Sch Mol & Biomed Sci, Adelaide, SA 5005, Australia

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2005年 / 43卷 / 06期

关键词：

D O I：

10.1128/JCM.43.6.2944-2947.2005

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We have recently described a novel AB(5) subtilase cytotoxin produced by certain Shiga toxigenic Escherichia coli (STEC) strains. This potentially lethal toxin may contribute to severe gastrointestinal and systemic disease in humans. In this study we have developed a trivalent PCR assay for the detection of the novel toxin A subunit gene subA, as well as stx(1) and str(2). The three primer pairs used in the assay do not interfere with each other and generate amplification products of 556, 180, and 255 bp, respectively. The assay can be used for determining the toxin genotype of STEC isolates, as well as for direct detection of toxin genes in primary fecal culture extracts.

引用

页码：2944 / 2947

页数：4

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