Regeneration of intervertebral discs in a rat disc degeneration model by implanted adipose-tissue-derived stromal cells

被引：146

作者：

Jeong, Je Hoon ^{[2
,3
]}

Lee, Jung Hwan ^{[3
,4
]}

Jin, Eun Sun ^{[3
,5
]}

Min, Joong Kee ^{[3
]}

Jeon, Sang Ryong ^{[3
]}

Choi, Kyoung Hyo ^{[1
,3
]}

机构：

[1] Univ Ulsan, Coll Med, Asan Med Ctr, Dept Rehabil Med, Seoul 138736, South Korea

[2] Hallym Univ, Coll Med, Dept Neurol Surg, Seoul, South Korea

[3] Univ Ulsan, Coll Med, Asan Med Ctr, Lab Stem Cell Therapy, Seoul 138736, South Korea

[4] Wooridul Spine Hosp, Dept Phys Med & Rehabil, Seoul, South Korea

[5] Kyung Hee EW Neo Med Ctr, Ctr Cardiovasc, Seoul, South Korea

来源：

ACTA NEUROCHIRURGICA | 2010年 / 152卷 / 10期

基金：

新加坡国家研究基金会;

关键词：

Adipose-tissue-derived stromal cell; Animal model; Intervertebral disc; Degeneration; MESENCHYMAL STEM-CELLS; BONE-MARROW; EXTRACELLULAR-MATRIX; IN-VIVO; THERAPY; CORD;

D O I：

10.1007/s00701-010-0698-2

中图分类号：

R74 [神经病学与精神病学];

学科分类号：

100204 [神经病学];

摘要：

Because adipose-tissue-derived stromal cell (ADSC) is readily accessible and abundant in stem cell, ADSC may be a better candidate for cell therapy and tissue engineering. This study investigated the potential of ADSC implantation to restore disc in a rat IVD model. The first coccygeal disc segments of a Sprague-Dawley rat was left undamaged as a control (NC) group, and other two segments were damaged by needle injection. Two weeks later, ADSCs (TS) group or saline (IN) group was transplanted into each of the two damaged segments. At 6 weeks after transplantation, the TS group showed a significantly smaller reduction in disc height than the IN group and exhibited a restoration of MRI signal intensity. Hematoxylin and eosin staining revealed a greater restoration of the inner annulus structure in the TS group. Anti-Human Nucleic Antibody, collagen type II, and aggrecan, staining showed positive findings at 2 weeks after transplantation in TS group. ADSCs show potential for restoring degenerative discs and may prove effective in the treatment of IVD.

引用

页码：1771 / 1777

页数：7

共 21 条

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