New zwitterionic detergents improve the analysis of membrane proteins by two-dimensional electrophoresis

被引：245

作者：

Chevallet, M

Santoni, V

Poinas, A

Rouquié, D

Fuchs, A

Kieffer, S

Rossignol, M

Lunardi, J

Garin, J

Rabilloud, T

机构：

[1] CEA Grenoble, DBMS, BECP, Lab Bioenerget Cellularie & Pathol, F-38054 Grenoble 9, France

[2] CNRS URA 573, Lab Biochim & Physiol Mol Plantes, Montpellier, France

[3] CEA, Lab Biol & Biochim Syst Integres, Grenoble, France

[4] CEA, Lab Chim Prot, Grenoble, France

来源：

ELECTROPHORESIS | 1998年 / 19卷 / 11期

关键词：

membrane proteins; protein solubility; two-dimensional polyacrylamide gel electrophoresis;

D O I：

10.1002/elps.1150191108

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Severe quantitative loss of protein is often observed in high-resolution two-dimensional electrophoresis of membrane proteins, while the resolution is usually not affected. To improve the solubility of proteins in this technique, we tested denaturing cocktails containing various detergents and chaotropes. Best results were obtained with a denaturing solution containing urea, thiourea, and zwitterionic detergents, synthesized for this purpose. Among the dozen detergents synthesized and tested, amidosulfobetaines with an alkyl tail containing 14-16 carbons proved most efficient, solubilizing previously undetected membrane proteins.

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页码：1901 / 1909

页数：9

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