Identification of Suitable Reference Genes for qRT-PCR Analysis of Circulating microRNAs in Hepatitis B Virus-Infected Patients

被引：59

作者：

Zhu, Hai-Tao ^{[1
,2
,3
]}

Dong, Qiong-Zhu ^{[1
,2
,3
]}

Wang, Guan ^{[1
,2
,3
]}

Zhou, Hai-Jun ^{[1
,2
,3
]}

Ren, Ning ^{[1
,2
,3
]}

Jia, Hu-Liang ^{[1
,2
,3
]}

Ye, Qing-Hai ^{[1
,2
,3
]}

Qin, Lun-Xiu ^{[1
,2
,3
]}

机构：

[1] Fudan Univ, Live Canc Inst, Shanghai 200032, Peoples R China

[2] Fudan Univ, Zhongshan Hosp, Inst Biomed Sci, Shanghai 200032, Peoples R China

[3] Minist Educ, Key Lab Carcinogenesis & Canc Invas, Shanghai, Peoples R China

来源：

MOLECULAR BIOTECHNOLOGY | 2012年 / 50卷 / 01期

基金：

中国国家自然科学基金;

关键词：

Hepatitis B virus; Circulating microRNA; Quantitative real-time PCR; Reference genes; Normalization; ACUTE MYOCARDIAL-INFARCTION; POTENTIAL BIOMARKER; CANCER; NORMALIZATION; EXPRESSION; PLASMA; DIAGNOSIS;

D O I：

10.1007/s12033-011-9414-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Circulating microRNAs (miRNAs) were found to exist in serum/plasma in a highly stable, cell-free form, and aberrantly expressed in many human diseases. Currently, the expression levels of circulating miRNAs are estimated by quantitative real-time polymerase chain reaction. However, no study has systematically evaluated reference genes for evaluating circulating microRNA expression. This study describes the identification and characterization of an appropriate reference gene for the normalization of circulating miRNA levels in hepatitis B virus (HBV)-infected patients and healthy people. Ten miRNAs that resemble the mean expression of the TaqMan low density array together with U6, RNU6B, and miR-16 were validated with two algorithms, geNorm, and NormFinder, after ensuring their equivalent expression between the two study groups. The combination of miR-26a, miR-221, and miR-22* is recommended as the most stable set of reference genes for circulating miRNA evaluation in HBV patients and healthy people.

引用

页码：49 / 56

页数：8

共 29 条

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