Sensitive gas chromatographic method for determination of mercapturic acids in human urine

被引：39

作者：

Einig, T ^{[1
]}

Dunemann, L ^{[1
]}

Dehnen, W ^{[1
]}

机构：

[1] MED INST UMWELTHYG,BIOCHEM ABT,D-40225 DUSSELDORF,GERMANY

来源：

JOURNAL OF CHROMATOGRAPHY B | 1996年 / 687卷 / 02期

关键词：

mercapturic acids; S-phenylmercapturic acid; S-benzlmercapturic acid;

D O I：

10.1016/S0378-4347(96)00259-9

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A method was developed for sensitive determination of the specific benzene metabolite S-phenylmercapturic acid and the corresponding toluene metabolite S-benzylmercapturic acid in human urine for non-occupational and occupational exposure. The sample preparation procedure consists of liquid extraction of urine samples followed by precolumn derivatization and a clean-up by normal-phase HPLC. Determination of analytes occurs by gas chromatography with electron-capture detection. With this highly sensitive method (detection limits 60 and 65 ng/l, respectively) urinary S-phenylmercapturic and S-benzylmercapturic acid concentrations for non-occupationally exposed persons (e.g. non-smokers) can be measured precisely in one chromatographic run. Validation of the method occurred by comparison with a HPLC method we have published recently.

引用

页码：379 / 385

页数：7

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