Effect of chitosan on membrane permeability and cell morphology of Pseudomonas aeruginosa and Staphyloccocus aureus

被引：105

作者：

Tao, Yan ^{[1
]}

Qian, Li-Hong ^{[1
]}

Xie, Jing ^{[1
]}

机构：

[1] Shanghai Ocean Univ, Coll Food Sci & Technol, Shanghai 201306, Peoples R China

来源：

CARBOHYDRATE POLYMERS | 2011年 / 86卷 / 02期

关键词：

Chitosan; Antibacterial activity; Antibacterial mechanism; Membrane permeability; ANTIBACTERIAL ACTIVITY; ESCHERICHIA-COLI; STAPHYLOCOCCUS-AUREUS; ANTIMICROBIAL PROPERTIES; KILLS BACTERIA; IN-VITRO; MECHANISM; PEPTIDES; PAPAIN; DAMAGE;

D O I：

10.1016/j.carbpol.2011.05.054

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

The mechanism of action of over 85% deacetylated chitosan on Gram-negative Pseudomonas aeruginosa and Gram-positive Staphylococcus aureus was investigated. Results showed that after treated with chitosan, the electric conductivity of bacteria suspensions increased, followed by increasing of the units of average release for alkaline phosphatase (ALP) and glucose-6-phosphate dehydrogenase (G6PDH). SOS-PAGE and agarose gel electrophoresis indicated that the soluble proteins and intact DNAs decreased or disappeared in chitosan-treated bacteria cells, demonstrating that chitosan performed its antibacterial function via increasing the permeability of cell membranes. Moreover. chitosan had a stronger effect on the cell membrane of S. aureus than on that of P. aeruginosa due to the differences in their cell structures. Transmission electron microscopy (TEM) observations further verified that S. aureus is more sensitive than P. aeruginosa to chitosan. Our works provide additional evidences in support of chitosan being regarded as a natural bactericide. Crown Copyright (C) 2011 Published by Elsevier Ltd. All rights reserved.

引用

页码：969 / 974

页数：6

共 34 条

[1] Determination of minimum inhibitory concentrations [J].

Andrews, JM .

JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 2001, 48 :5-16

[2] Effect of water-soluble reduced chitosan on Streptococcus mutans, plaque regrowth and biofilm vitality [J].

Bae, K. ;

Jun, E. J. ;

Lee, S. M. ;

Paik, D. I. ;

Kim, J. B. .

CLINICAL ORAL INVESTIGATIONS, 2006, 10 (02) :102-107

[3] Structural and antimicrobial properties of human pre-elafin/trappin-2 and derived peptides against Pseudomonas aeruginosa [J].

Bellemare, Audrey ;

Vernoux, Nathalie ;

Morin, Sebastien ;

Gagne, Stephane M. ;

Bourbonnais, Yves .

BMC MICROBIOLOGY, 2010, 10

[4] Investigation of functional and morphological changes in Pseudomonas aeruginosa and Staphylococcus aureus cells induced by Origanum compactum essential oil [J].

Bouhdid, S. ;

Abrini, J. ;

Zhiri, A. ;

Espuny, M. J. ;

Manresa, A. .

JOURNAL OF APPLIED MICROBIOLOGY, 2009, 106 (05) :1558-1568

[5] Antibacterial effects of N-sulfonated and N-sulfobenzoyl chitosan and application to oyster preservation [J].

Chen, CS ;

Liau, WY ;

Tsai, GJ .

JOURNAL OF FOOD PROTECTION, 1998, 61 (09) :1124-1128

[6] Factors affecting the susceptibility of Staphylococcus aureus CCRC 12657 to water soluble lactose chitosan derivative [J].

Chen, YL ;

Chou, CC .

FOOD MICROBIOLOGY, 2005, 22 (01) :29-35

[7] Effect of abiotic factors on the antibacterial activity of chitosan against waterborne pathogens [J].

Chung, YC ;

Wang, HL ;

Chen, YM ;

Li, SL .

BIORESOURCE TECHNOLOGY, 2003, 88 (03) :179-184

[8] N-CARBOXYMETHYLCHITOSAN INHIBITION OF AFLATOXIN PRODUCTION - ROLE OF ZINC [J].

CUERO, RG ;

OSUJI, G ;

WASHINGTON, A .

BIOTECHNOLOGY LETTERS, 1991, 13 (06) :441-444

[9] Mechanism of action of neomycin on Erwinia carotovora subsp carotovora [J].

Cui, Wenhua ;

Quan, Xin ;

Tao, Ke ;

Teng, Yun ;

Zhang, Xingang ;

Liu, Yun ;

Shi, Guanying ;

Hou, Taiping .

PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY, 2009, 95 (02) :85-89

[10] Chitosan disrupts the barrier properties of the outer membrane of Gram-negative bacteria [J].

Helander, IM ;

Nurmiaho-Lassila, EL ;

Ahvenainen, R ;

Rhoades, J ;

Roller, S .

INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 2001, 71 (2-3) :235-244

← 1 2 3 4 →