Expression profiling reveals off-target gene regulation by RNAi

被引：1753

作者：

Jackson, AL ^{[1
]}

Bartz, SR ^{[1
]}

Schelter, J ^{[1
]}

Kobayashi, SV ^{[1
]}

Burchard, J ^{[1
]}

Mao, M ^{[1
]}

Li, B ^{[1
]}

Cavet, G ^{[1
]}

Linsley, PS ^{[1
]}

机构：

[1] Rosetta Inpharmat LLC, Kirkland, WA 98034 USA

来源：

NATURE BIOTECHNOLOGY | 2003年 / 21卷 / 06期

关键词：

D O I：

10.1038/nbt831

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

RNA interference is thought to require near-identity between the small interfering RNA (siRNA) and its cognate mRNA. Here, we used gene expression profiling to characterize the specificity of gene silencing by siRNAs in cultured human cells. Transcript profiles revealed siRNA-specific rather than target-specific signatures, including direct silencing of nontargeted genes containing as few as eleven contiguous nucleotides of identity to the siRNA. These results demonstrate that siRNAs may cross-react with targets of limited sequence similarity.

引用

页码：635 / 637

页数：3

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