Rice gene expression in response to N-acetylchitooligosaccharide elicitor:: comprehensive analysis by DNA microarray with randomly selected ESTs

被引：52

作者：

Akimoto-Tomiyama, C

Sakata, K

Yazaki, J

Nakamura, K

Fujii, F

Shimbo, K

Yamamoto, K

Sasaki, T

Kishimoto, N

Kikuchi, S

Shibuya, N

Minami, E

机构：

[1] Natl Inst Agrobiol Sci, Dept Biochem, Tsukuba, Ibaraki 3058602, Japan

[2] Natl Inst Agrobiol Sci, Dept Genome Res, Tsukuba, Ibaraki 3058602, Japan

[3] Natl Inst Agrobiol Sci, Dept Mol Genet, Tsukuba, Ibaraki 3058602, Japan

[4] STAFF Inst, Tsukuba, Ibaraki 3050854, Japan

来源：

PLANT MOLECULAR BIOLOGY | 2003年 / 52卷 / 03期

关键词：

CDPK; microarray; N-acetylchitooligosaccharides; Oryza sativa; rice cells;

D O I：

10.1023/A:1024890601888

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

N-acetylchitooligosaccharides are potent elicitors to suspension-cultured rice cells, inducing a set of defense reactions. Expression of defense-related genes is considered to play an important role in defense reactions, and we employed microarray analysis of 8987 randomly selected expressed sequence tags to analyze the changes in gene expression caused by N-acetylchitooctaose. In this experiment, 166 genes were significantly induced and 93 genes were repressed. RNA gel blot analysis of 16 of these genes confirmed the microarray results. Of the 259 ESTs identified as responsive to N-acetylchytooctaose, 18 genes are related to signal transduction, including five calcium-dependent protein kinases (CDPKs). Among these, three novel CDPKs responsive to N-acetylchitooctaose were isolated.

引用

页码：537 / 551

页数：15

共 41 条

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