Electrophoretic mobility of composite objects in free solution: Application to DNA separation

被引：48

作者：

Long, D ^{[1
]}

Ajdari, A ^{[1
]}

机构：

[1] ECOLE SUPER PHYS & CHIM IND VILLE PARIS,URA CNRS 1382,LAB PHYSICOCHIM THEOR,F-75231 PARIS 05,FRANCE

来源：

ELECTROPHORESIS | 1996年 / 17卷 / 06期

关键词：

free-solution electrophoresis; capillary electrophoresis; end-labeled free-solution electrophoresis; polyelectrolyte; DNA;

D O I：

10.1002/elps.1150170628

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We propose here a simple procedure to estimate the electrophoretic mobility of composite objects obtained by linking two charged subunits, stressing that this electrophoretic mobility is in general different from the ratio of the total charge to the total friction coefficient. We focus especially on the situation where at least one of the subunits is a polyelectrolyte. Our remarks in particular correct the existing theoretical analysis of separation capabilities of devices in which a buoy (sphere, protein, polymer) is attached to a DNA chain. We also predict that, in some cases, the direction of electrophoretic motion can be reversed by increasing the electric field amplitude.

引用

页码：1161 / 1166

页数：6

共 33 条

[1]

AJDARI A, 1994, PHYSICA A, V204, P17, DOI 10.1016/0378-4371(94)90415-4

[2] EFFECT OF NONUNIFORM ZETA POTENTIAL ON PARTICLE MOVEMENT IN ELECTRIC-FIELDS [J].