Evidence against a role for polymorphisms at tumor necrosis factor, interleukin-1 and interleukin-1 receptor antagonist gene loci in the regulation of disease severity in acute pancreatitis

被引:46
作者
Powell, JJ [1 ]
Fearon, KCH [1 ]
Siriwardena, AK [1 ]
Ross, JA [1 ]
机构
[1] Univ Edinburgh, Royal Infirm Edinburgh, Dept Surg & Clin Sci, Edinburgh EH3 9YW, Midlothian, Scotland
关键词
D O I
10.1067/msy.2001.113375
中图分类号
R61 [外科手术学];
学科分类号
摘要
Background. Cytokines such as tumor necrosis factor-alpha (TNF-alpha) and interleukin-1 beta (IL-1 beta) and their endogenous antagonists such as IL-l receptor antagonist (IL-1RA) are important mediators of disease severity in acute pancreatitis. Because the level of secretion of these cytokines is determined in part by genetic factors, the aim of this study: was to examine the influence of genetically determined cytokine secretion upon disease severity in acute pancreatitis. Methods. TNF (TNF-308, TNFB), IL-1 beta, and IL-1 receptor antagonist (IL-IRA) genotypes were determined for 190 patients with acute pancreatitis and 102 healthy volunteers. To further assess the influence of genetic factors, the cytokine phenotype for TNF-alpha, IL-1 beta, and IL-1RA was determined by using a whole blood culture technique in 51 patients after recovery. Results. The distributions of TNF-308, TNFB, IL-1 beta, and IL-1RA gene polymorphisms were similar in patients with mild or severe acute pancreatitis. Further, no difference in gene polymorphism frequencies was observed between patients with acute pancreatitis and healthy controls. With respect to phenotype, the secretion of TNF-alpha was similar in patients with previous mild and severe acute pancreatitis; however, the IL-1 beta: IL-1RA ratio was significantly lower in patients with previous severe acute pancreatitis than in those with mild disease. Conclusions. Our observations suggest that genetic factors are not important in determining TNF-alpha secretion in patients with acute pancreatitis. However; a predetermined imbalance between IL-1 beta and its antagonist IL-1RA would appear to exist in patients with severe acute pancreatitis, although the genetic basis for this altered relationship could not be determined.
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页码:633 / 640
页数:8
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