Cryopreservation of potato shoot tips by encapsulation-dehydration

被引：45

作者：

Bouafia, S ^{[1
]}

Jelti, N ^{[1
]}

Lairy, G ^{[1
]}

Blanc, A ^{[1
]}

Bonnel, E ^{[1
]}

Dereuddre, J ^{[1
]}

机构：

[1] KERGUIVARCH,GERMICOPA,F-29119 CHATEAUNEUF DU FA,FRANCE

来源：

POTATO RESEARCH | 1996年 / 39卷 / 01期

关键词：

Solanum; preculture; liquid nitrogen;

D O I：

10.1007/BF02358208

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

Potato shoot tips excised from 2-week-old in vitro nodal cuttings were cryopreserved after encapsulation in alginate beads. Encapsulated shoot tips were first precultured in sucrose-enriched media, dried over silica gel, and rapidly cooled in liquid nitrogen. After slow rewarming in air at room temperature, alginate beads were transferred to solid culture medium for shoot tip recovery. After cooling in liquid nitrogen, shoot yield depended on preculture duration, sucrose concentration and water content of beads. Survival rates above 60% were obtained for each cultivar tested.

引用

页码：69 / 78

页数：10

共 29 条

[1]

BAJAJ Y P S, 1977, Crop Improvement, V4, P48

[2]

BAJAJ Y P S, 1978, Crop Improvement, V5, P137

[3]

BAJAJ YPS, 1985, INDIAN J EXP BIOL, V23, P285

[4]

BENSON EE, 1989, CRYO-LETT, V10, P323

[5]

DEREUDDRE J, 1990, CR ACAD SCI III-VIE, V310, P317

[6] RESISTANCE TO FREEZING IN LIQUID-NITROGEN OF CARNATION (DIANTHUS-CARYOPHYLLUS L VAR EOLO) APICAL AND AXILLARY SHOOT TIPS EXCISED FROM DIFFERENT AGED INVITRO PLANTLETS [J].

DEREUDDRE, J ;

FABRE, J ;

BASSAGLIA, C .

PLANT CELL REPORTS, 1988, 7 (03) :170-173

[7]

DEREUDDRE J, 1991, CRYOLETTERS, V12, P135

[8]

FABRE J, 1990, CRYO-LETT, V11, P413

[9] FREEZE PRESERVATION OF POTATO SHOOT-TIP CULTURES [J].

GROUT, BWW ;

HENSHAW, GG .

ANNALS OF BOTANY, 1978, 42 (181) :1227-1229

[10]

HARDING K, 1991, CRYO-LETT, V12, P17

← 1 2 3 →